RRC ID 48816
Author Shirozu M, Nakano T, Inazawa J, Tashiro K, Tada H, Shinohara T, Honjo T.
Title Structure and chromosomal localization of the human stromal cell-derived factor 1 (SDF1) gene.
Journal Genomics
Abstract Stromal cell-derived factors 1 alpha and 1 beta are small cytokines belonging to the intercrine CXC subfamily and originally isolated from a murine bone-marrow stroma cell line by the signal sequence trap method. cDNA and genomic clones of human SDF1 alpha and SDF1 beta (SDF1A and SDF1B) were isolated and characterized. cDNAs of SDF1 alpha and SDF1 beta encode proteins of 89 and 93 amino acids, respectively. SDF1 alpha and SDF1 beta sequences are more than 92% identical to those of the human counterparts. The genomic structure of the SDF1 gene revealed that human SDF1 alpha and SDF1 beta are encoded by a single gene and arise by alternative splicing. SDF1 alpha and SDF1 beta are encoded by 3 and 4 exons, respectively. Ubiquitous expression of the SDF1 gene, except in blood cells, was consistent with the presence of the GC-rich sequence in the 5'-flanking region of the SDF1 gene, as is often the case in the "housekeeping" genes. Although genes encoding other members of the intercrine family are localized on chromosome 4q or 17q, the human SDF1 gene was mapped to chromosome 10q by fluorescence in situ hybridization. Strong evolutionary conservation and unique chromosomal localization of the SDF1 gene suggest that SDF1 alpha and SDF1 beta may have important functions distinct from those of other members of the intercrine family.
Volume 28(3)
Pages 495-500
Published 1995-8-10
DOI 10.1006/geno.1995.1180
PII S0888-7543(85)71180-9
PMID 7490086
MeSH Amino Acid Sequence Animals Base Sequence Biological Evolution Chemokine CXCL12 Chemokines, CXC* Chickens Chromosome Mapping Chromosomes, Human, Pair 10* Cloning, Molecular Conserved Sequence Cytokines / genetics* DNA DNA, Complementary / analysis Humans Mice Molecular Sequence Data Rabbits Xenopus
IF 6.205
Times Cited 514
DNA material human SDF1 genomic clone 4 (RDB06780) human SDF1 genomic clone 6 (RDB06781) human SDF-1 alpha cDNA (RDB07035) human SDF-1 beta cDNA (RDB07036).