Reference - Detail
| RRC ID | 48820 |
|---|---|
| Author | Matsunami N, Hamaguchi Y, Yamamoto Y, Kuze K, Kangawa K, Matsuo H, Kawaichi M, Honjo T. |
| Title | A protein binding to the J kappa recombination sequence of immunoglobulin genes contains a sequence related to the integrase motif. |
| Journal | Nature |
| Abstract |
Site-specific recombination requires conserved DNA sequences specific to each system, and system-specific proteins that recognize specific DNA sequences. The site-specific recombinases seem to fall into at least two families, based on their protein structure and chemistry of strand breakage. One of these is the resolvase-invertase family, members of which seem to form a serine-phosphate linkage with DNA. Members of the other family, called the integrase family, contain a conserved tyrosine residue that forms a covalent linkage with the 3'-phosphate of DNA at the site of recombination. Structural comparison of integrases shows that these proteins share a highly conserved 40-residue motif. V-(D)-J recombination of the immunoglobulin gene requires conserved recombination signal sequences (RS) of a heptamer CACTGTG and a T-rich nonamer GGTTTTTGT, which are separated by a spacer sequence of either 12 or 23 bases We have recently purified, almost to homogeneity, a protein that specifically binds to the immunoglobulin J kappa RS containing the 23-base-pair spacer sequence. By synthesizing probes on the basis of partial amino-acid sequences of the purified protein, we have now isolated and characterized the complementary DNA of this protein. The amino-acid sequence deduced from the cDNA sequence reveals that the J kappa RS-binding protein has a sequence similar to the 40-residue motif of integrases of phages, bacteria and yeast, indicating that this protein could be involved in V-(D)-J recombination as a recombinase. |
| Volume | 342(6252) |
| Pages | 934-7 |
| Published | 1989-12-21 |
| DOI | 10.1038/342934a0 |
| PMID | 2556644 |
| MeSH | Amino Acid Sequence Animals B-Lymphocytes / immunology Base Sequence Cell Line DNA Nucleotidyltransferases / genetics* DNA Transposable Elements DNA-Binding Proteins / genetics DNA-Binding Proteins / isolation & purification Gene Rearrangement Genes, Immunoglobulin* Immunoglobulin kappa-Chains / genetics* Integrases Mice Molecular Sequence Data Plasmids Recombination, Genetic* Restriction Mapping Sequence Homology, Nucleic Acid |
| IF | 42.779 |
| Times Cited | 149 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | RBP-J (RDB01801) |