論文 - 詳細
| RRC ID | 48834 |
|---|---|
| 著者 | Feng R, Satoh Y, Ogasawara Y, Yoshimura T, Dairi T. |
| タイトル | A Glycopeptidyl-Glutamate Epimerase for Bacterial Peptidoglycan Biosynthesis. |
| ジャーナル | J Am Chem Soc |
| Abstract |
d-Glutamate (Glu) supplied by Glu racemases or d-amino acid transaminase is utilized for peptidoglycan biosynthesis in microorganisms. Comparative genomics has shown that some microorganisms, including Xanthomonas oryzae, perhaps have no orthologues of these genes. We performed shotgun cloning experiments with a d-Glu auxotrophic Escherichia coli mutant as the host and X. oryzae as the DNA donor. We obtained complementary genes, XOO_1319 and XOO_1320, which are annotated as a hypothetical protein and MurD (UDP-MurNAc-l-Ala-d-Glu synthetase), respectively. By detailed in vitro analysis, we revealed that XOO_1320 is an enzyme to ligate l-Glu to UDP-MurNAc-l-Ala, providing the first example of MurD utilizing l-Glu, and that XOO_1319 is a novel enzyme catalyzing epimerization of the terminal l-Glu of the product in the presence of ATP and Mg2+. We investigated the occurrence of XOO_1319 orthologues and found that it exists in some categories of microorganisms, including pathogenic ones. |
| 巻・号 | 139(12) |
| ページ | 4243-4245 |
| 公開日 | 2017-3-29 |
| DOI | 10.1021/jacs.7b01221 |
| PMID | 28294606 |
| MeSH | Gammaproteobacteria / chemistry Gammaproteobacteria / metabolism* Glutamic Acid / chemistry Glutamic Acid / metabolism* Glycopeptides / chemistry Glycopeptides / metabolism* Peptidoglycan / biosynthesis* Peptidoglycan / chemistry Racemases and Epimerases / metabolism* |
| IF | 14.612 |
| 引用数 | 6 |
| WOS 分野 | CHEMISTRY, MULTIDISCIPLINARY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | Genomic DNA of Stenotrophomonas maltophilia JCM 1975T (JGD12766) |
| 一般微生物 | |