RRC ID 48834
著者 Feng R, Satoh Y, Ogasawara Y, Yoshimura T, Dairi T.
タイトル A Glycopeptidyl-Glutamate Epimerase for Bacterial Peptidoglycan Biosynthesis.
ジャーナル J Am Chem Soc
Abstract d-Glutamate (Glu) supplied by Glu racemases or d-amino acid transaminase is utilized for peptidoglycan biosynthesis in microorganisms. Comparative genomics has shown that some microorganisms, including Xanthomonas oryzae, perhaps have no orthologues of these genes. We performed shotgun cloning experiments with a d-Glu auxotrophic Escherichia coli mutant as the host and X. oryzae as the DNA donor. We obtained complementary genes, XOO_1319 and XOO_1320, which are annotated as a hypothetical protein and MurD (UDP-MurNAc-l-Ala-d-Glu synthetase), respectively. By detailed in vitro analysis, we revealed that XOO_1320 is an enzyme to ligate l-Glu to UDP-MurNAc-l-Ala, providing the first example of MurD utilizing l-Glu, and that XOO_1319 is a novel enzyme catalyzing epimerization of the terminal l-Glu of the product in the presence of ATP and Mg2+. We investigated the occurrence of XOO_1319 orthologues and found that it exists in some categories of microorganisms, including pathogenic ones.
巻・号 139(12)
ページ 4243-4245
公開日 2017-3-29
DOI 10.1021/jacs.7b01221
PMID 28294606
MeSH Gammaproteobacteria / chemistry Gammaproteobacteria / metabolism* Glutamic Acid / chemistry Glutamic Acid / metabolism* Glycopeptides / chemistry Glycopeptides / metabolism* Peptidoglycan / biosynthesis* Peptidoglycan / chemistry Racemases and Epimerases / metabolism*
IF 14.612
引用数 6
WOS 分野 CHEMISTRY, MULTIDISCIPLINARY
リソース情報
遺伝子材料 Genomic DNA of Stenotrophomonas maltophilia JCM 1975T (JGD12766)
一般微生物