RRC ID 4915
Author Brouns SJ, Jore MM, Lundgren M, Westra ER, Slijkhuis RJ, Snijders AP, Dickman MJ, Makarova KS, Koonin EV, van der Oost J.
Title Small CRISPR RNAs guide antiviral defense in prokaryotes.
Journal Science
Abstract Prokaryotes acquire virus resistance by integrating short fragments of viral nucleic acid into clusters of regularly interspaced short palindromic repeats (CRISPRs). Here we show how virus-derived sequences contained in CRISPRs are used by CRISPR-associated (Cas) proteins from the host to mediate an antiviral response that counteracts infection. After transcription of the CRISPR, a complex of Cas proteins termed Cascade cleaves a CRISPR RNA precursor in each repeat and retains the cleavage products containing the virus-derived sequence. Assisted by the helicase Cas3, these mature CRISPR RNAs then serve as small guide RNAs that enable Cascade to interfere with virus proliferation. Our results demonstrate that the formation of mature guide RNAs by the CRISPR RNA endonuclease subunit of Cascade is a mechanistic requirement for antiviral defense.
Volume 321(5891)
Pages 960-4
Published 2008-8-15
DOI 10.1126/science.1159689
PII 321/5891/960
PMID 18703739
PMC PMC5898235
MeSH Amino Acid Sequence Bacteriophage lambda / genetics* Bacteriophage lambda / growth & development* Base Sequence DNA, Intergenic DNA, Viral / metabolism Escherichia coli / genetics Escherichia coli / metabolism Escherichia coli K12 / genetics* Escherichia coli K12 / metabolism Escherichia coli K12 / virology* Escherichia coli Proteins / chemistry Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Genes, Bacterial Molecular Sequence Data RNA Precursors / metabolism RNA, Bacterial / genetics* RNA, Bacterial / metabolism RNA, Guide / genetics RNA, Guide / metabolism Repetitive Sequences, Nucleic Acid* Transcription, Genetic Viral Plaque Assay
IF 41.846
Times Cited 1202
Prokaryotes E. coli CV-4(BL21(DE3)) W3110 Keio collection