RRC ID 49248
Author Yang Z, Liu M, Wang W, Wang Y, Cao B, Gao Y, Chen H, Li T.
Title Pseudolaric acid B attenuates atopic dermatitis-like skin lesions by inhibiting interleukin-17-induced inflammation.
Journal Sci Rep
Abstract Pseudolaric acid B (PB), isolated from the extract of the root bark of Pseudolarix kaempferi Gordon, has been used as a traditional remedy for the treatment of skin diseases. However, the information of PB on atopic dermatitis (AD) remains largely unknown. In the present study, oral administration with PB improved the severity scores of AD-like skin lesions dose-dependently in NC/Nga mice through reducing serum IgE, pro-inflammatory cytokines, and the infiltration of inflammatory cells. In addition, PB significantly attenuated the levels of IL-17 and IL-22, and the proportion of Th17 cells in NC/Nga mice, as well as decreased IL-17-induced inflammation in RAW264.7 cells. Moreover, PB inhibited the phosphorylation of IκBα and miR-155 expression both in NC/Nga mice and in IL-17-stimulated RAW264.7 cells, which could be reversed by GW9662, a specific antagonist for PPARγ. The incorporation of GW9662 reversed the inhibitory effect of PB on the RORγ-mediated activation of the Il17 promoter. Transfection with PPARγ luciferase reporter gene further demonstrated the enhancement of PB on PPARγ transactivation. These findings indicate that PB could ameliorate AD-like skin lesions by inhibiting IL-17-induced inflammation in a PPARγ-dependent manner, which would provide experimental evidence of PB for the therapeutic potential on AD and other inflammatory skin diseases.
Volume 7(1)
Pages 7918
Published 2017-8-11
DOI 10.1038/s41598-017-08485-x
PII 10.1038/s41598-017-08485-x
PMID 28801611
PMC PMC5554149
MeSH Animals Anti-Inflammatory Agents / administration & dosage* Dermatitis, Atopic / drug therapy* Dermatitis, Atopic / pathology Disease Models, Animal Diterpenes / administration & dosage* Interleukin-17 / antagonists & inhibitors* Macrophages / drug effects Macrophages / immunology Mice RAW 264.7 Cells Th17 Cells / drug effects Th17 Cells / immunology
IF 3.998
Times Cited 5
Mice RBRC01059