論文 - 詳細
| RRC ID | 49251 |
|---|---|
| 著者 | Watanabe K, Kobayashi A, Endo M, Sage-Ono K, Toki S, Ono M. |
| タイトル | CRISPR/Cas9-mediated mutagenesis of the dihydroflavonol-4-reductase-B (DFR-B) locus in the Japanese morning glory Ipomoea (Pharbitis) nil. |
| ジャーナル | Sci Rep |
| Abstract |
CRISPR/Cas9 technology is a versatile tool for targeted mutagenesis in many organisms, including plants. However, this technique has not been applied to the Japanese morning glory (Ipomoea [Pharbitis] nil), a traditional garden plant chosen for the National BioResource Project in Japan. We selected dihydroflavonol-4-reductase-B (DFR-B) of I. nil, encoding an anthocyanin biosynthesis enzyme, as the target gene, and changes in the stem colour were observed during the early stages of plant tissue culture by Rhizobium [Agrobacterium]-mediated transformation. Twenty-four of the 32 (75%) transgenic plants bore anthocyanin-less white flowers with bi-allelic mutations at the Cas9 cleavage site in DFR-B, exhibiting a single base insertion or deletions of more than two bases. Thus, these results demonstrate that CRISPR/Cas9 technology enables the exploration of gene functions in this model horticultural plant. To our knowledge, this report is the first concerning flower colour changes in higher plants using CRISPR/Cas9 technology. |
| 巻・号 | 7(1) |
| ページ | 10028 |
| 公開日 | 2017-8-30 |
| DOI | 10.1038/s41598-017-10715-1 |
| PII | 10.1038/s41598-017-10715-1 |
| PMID | 28855641 |
| PMC | PMC5577235 |
| MeSH | Alcohol Oxidoreductases / genetics* CRISPR-Cas Systems* Ipomoea / enzymology Ipomoea / genetics* Mutagenesis* Plant Proteins / genetics* |
| IF | 3.998 |
| 引用数 | 22 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 55 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| アサガオ | Violet |