RRC ID |
49251
|
著者 |
Watanabe K, Kobayashi A, Endo M, Sage-Ono K, Toki S, Ono M.
|
タイトル |
CRISPR/Cas9-mediated mutagenesis of the dihydroflavonol-4-reductase-B (DFR-B) locus in the Japanese morning glory Ipomoea (Pharbitis) nil.
|
ジャーナル |
Sci Rep
|
Abstract |
CRISPR/Cas9 technology is a versatile tool for targeted mutagenesis in many organisms, including plants. However, this technique has not been applied to the Japanese morning glory (Ipomoea [Pharbitis] nil), a traditional garden plant chosen for the National BioResource Project in Japan. We selected dihydroflavonol-4-reductase-B (DFR-B) of I. nil, encoding an anthocyanin biosynthesis enzyme, as the target gene, and changes in the stem colour were observed during the early stages of plant tissue culture by Rhizobium [Agrobacterium]-mediated transformation. Twenty-four of the 32 (75%) transgenic plants bore anthocyanin-less white flowers with bi-allelic mutations at the Cas9 cleavage site in DFR-B, exhibiting a single base insertion or deletions of more than two bases. Thus, these results demonstrate that CRISPR/Cas9 technology enables the exploration of gene functions in this model horticultural plant. To our knowledge, this report is the first concerning flower colour changes in higher plants using CRISPR/Cas9 technology.
|
巻・号 |
7(1)
|
ページ |
10028
|
公開日 |
2017-8-30
|
DOI |
10.1038/s41598-017-10715-1
|
PII |
10.1038/s41598-017-10715-1
|
PMID |
28855641
|
PMC |
PMC5577235
|
MeSH |
Alcohol Oxidoreductases / genetics*
CRISPR-Cas Systems*
Ipomoea / enzymology
Ipomoea / genetics*
Mutagenesis*
Plant Proteins / genetics*
|
IF |
3.998
|
引用数 |
22
|
リソース情報 |
アサガオ |
Violet |