論文 - 詳細
| RRC ID | 49282 |
|---|---|
| 著者 | Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. |
| タイトル | Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. |
| ジャーナル | Chem Biol |
| Abstract |
The APC(Cdh1) E3 ligase is active in the late M and G(1) phases. Geminin is a direct substrate of the APC(Cdh1) complex, and accumulates during the S, G(2), and M phases. By fusing the amino-terminal region of Geminin to fluorescent proteins, we have developed cell cycle markers that accumulate in the S/G(2)/M phases in both the nucleus and the cytoplasm. These markers reveal the morphology of individual cells that have undergone DNA replication, allowing us to monitor cell growth relative to differentiation of various cell types. After electroporating the developing mouse embryos, we highlighted neuroepithelial progenitors in the S/G(2)/M phases, which possessed an elongated morphology with an apical and/or a basal attachment. We also show that nuclear localization of the ubiquitin ligase for Geminin is essential for full performance of the markers. |
| 巻・号 | 15(12) |
| ページ | 1243-8 |
| 公開日 | 2008-12-22 |
| DOI | 10.1016/j.chembiol.2008.10.015 |
| PII | S1074-5521(08)00420-1 |
| PMID | 19101468 |
| MeSH | Biomarkers / metabolism Cell Cycle* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Division / physiology* Cell Nucleus / metabolism* Cytoplasm / metabolism Flow Cytometry Fluorescence* G2 Phase / physiology* Geminin Genetic Vectors / genetics HeLa Cells Humans Lentivirus / genetics S Phase / physiology* |
| IF | 5.915 |
| 引用数 | 43 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | mVenus-hGeminin(1/110)/pcDNA3 (RDB15458) mVenus-hGeminin(1/110) / pCSII-EF-MCS (RDB15271) mCherry-hGeminin(1/110) / pCSII-EF-MCS (RDB15269) mVenus-hGeminin(1/60) / pCSII-EF-MCS (RDB15272) mCherry-hGeminin(1/60) / pCSII-EF-MCS (RDB15270). |