| RRC ID |
49284
|
| Author |
Itoh A, Fukata Y, Miyanaka H, Nonaka Y, Ogawa T, Nakamura T, Nishi N.
|
| Title |
Optimization of the inter-domain structure of galectin-9 for recombinant production.
|
| Journal |
Glycobiology
|
| Abstract |
We previously developed a stable form of galectin-9, an immunomodulatory animal lectin with a truncated linker peptide (G9Null), to overcome the protease sensitivity of wild-type galectin-9. G9Null is highly resistant to proteolysis, while the modification marginally improved the low solubility of the wild-type protein. To increase its solubility, we further modified the remaining linker region of G9Null. A 10-amino acid deletion with a single amino acid substitution resulted in an ∼400% increase in solubility and yield without an adverse effect on its biological activity. This mutant protein might be useful for large-scale recombinant production needed for evaluation of the therapeutic potential of galectin-9.
|
| Volume |
23(8)
|
| Pages |
920-5
|
| Published |
2013-8-1
|
| DOI |
10.1093/glycob/cwt023
|
| PII |
cwt023
|
| PMID |
23507964
|
| MeSH |
Amino Acid Substitution*
Animals
Galectins / biosynthesis
Galectins / chemistry
Galectins / genetics*
Protein Structure, Tertiary
Recombinant Fusion Proteins / biosynthesis
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / genetics
Solubility
|
| IF |
4.06
|
| Times Cited |
6
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
pET-ssG9 (RDB15282) |
