RRC ID 49299
Author Saito Y, Sugimura T, Yanagawa Y.
Title Comparisons of Neuronal and Excitatory Network Properties between the Rat Brainstem Nuclei that Participate in Vertical and Horizontal Gaze Holding.
Journal eNeuro
Abstract Gaze holding is primarily controlled by neural structures including the prepositus hypoglossi nucleus (PHN) for horizontal gaze and the interstitial nucleus of Cajal (INC) for vertical and torsional gaze. In contrast to the accumulating findings of the PHN, there is no report regarding the membrane properties of INC neurons or the local networks in the INC. In this study, to verify whether the neural structure of the INC is similar to that of the PHN, we investigated the neuronal and network properties of the INC using whole-cell recordings in rat brainstem slices. Three types of afterhyperpolarization (AHP) profiles and five firing patterns observed in PHN neurons were also observed in INC neurons. However, the overall distributions based on the AHP profile and the firing patterns of INC neurons were different from those of PHN neurons. The application of burst stimulation to a nearby site of a recorded INC neuron induced an increase in the frequency of spontaneous EPSCs. The duration of the increased EPSC frequency of INC neurons was not significantly different from that of PHN neurons. The percent of duration reduction induced by a Ca2+-permeable AMPA (CP-AMPA) receptor antagonist was significantly smaller in the INC than in the PHN. These findings suggest that local excitatory networks that activate sustained EPSC responses also exist in the INC, but their activation mechanisms including the contribution of CP-AMPA receptors differ between the INC and the PHN.
Volume 4(5)
Published 2017-9-13
DOI 10.1523/ENEURO.0180-17.2017
PII eN-NWR-0180-17
PMID 28966973
PMC PMC5616193
MeSH Animals Animals, Newborn Brain Stem / cytology* Brain Stem / metabolism* Cell Nucleus / drug effects Cell Nucleus / genetics Choline O-Acetyltransferase / genetics Choline O-Acetyltransferase / metabolism Electric Stimulation Excitatory Postsynaptic Potentials / genetics Fixation, Ocular / physiology* Glycine Agents / pharmacology Luminescent Proteins / genetics Luminescent Proteins / metabolism Nerve Net / drug effects Nerve Net / physiology* Neural Inhibition / drug effects Neural Inhibition / genetics Neurons / drug effects Neurons / physiology* Patch-Clamp Techniques Rats Rats, Transgenic Rats, Wistar Spermine / analogs & derivatives Spermine / pharmacology Strychnine / pharmacology Vesicular Inhibitory Amino Acid Transport Proteins / genetics Vesicular Inhibitory Amino Acid Transport Proteins / metabolism
IF 3.544
Times Cited 2
Rats W-Tg(Slc32a1-YFP*)2Yyan (strainID=908)