Reference - Detail
| RRC ID | 49304 |
|---|---|
| Author | Luo L, Siah CK, Cai Y. |
| Title | Engrailed acts with Nejire to control decapentaplegic expression in the Drosophila ovarian stem cell niche. |
| Journal | Development |
| Abstract |
Homeostasis of adult tissues is maintained by a small number of stem cells, which are sustained by their niches. In the Drosophila female germline stem cell (GSC) niche, Decapentaplegic (Dpp) is the primary factor that promotes GSC self-renewal. However, the mechanism regulating dpp expression in the niche is largely unknown. Here, we identify a 2.0 kb fragment located in a 5' cis-regulatory region of the dpp locus containing enhancer activity that drives its expression in the niche. This region is distinct from a previously characterized 3' cis-regulatory enhancer responsible for dpp expression in imaginal discs. Our data demonstrate that Engrailed, a homeodomain-containing transcription factor that serves as a cap cell marker, binds to this region and regulates dpp expression in cap cells. Further data suggest that En forms a complex with Nejire (Nej), the Drosophila ortholog of histone acetyltransferase CBP/p300, and directs Nej to this cis-regulatory region where Nej functions as the co-activator for dpp expression. Therefore, our study defines the molecular pathway controlling dpp expression in the Drosophila ovarian stem cell niche. |
| Volume | 144(18) |
| Pages | 3224-3231 |
| Published | 2017-9-15 |
| DOI | 10.1242/dev.145474 |
| PII | 144/18/3224 |
| PMID | 28928281 |
| MeSH | Animals Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / metabolism* Female Gene Expression Regulation Genes, Reporter Homeodomain Proteins / metabolism* Ovary / cytology* Ovary / metabolism* Protein Binding Regulatory Sequences, Nucleic Acid / genetics Stem Cell Niche* / genetics Stem Cells / cytology Stem Cells / metabolism Transcription Factors / metabolism* p300-CBP Transcription Factors / metabolism* |
| IF | 5.611 |
| Times Cited | 6 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | |