RRC ID 49307
著者 Nonaka S, Arai C, Takayama M, Matsukura C, Ezura H.
タイトル Efficient increase of ɣ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis.
ジャーナル Sci Rep
Abstract γ-Aminobutyric acid (GABA) is a non-proteinogenic amino acid that has hypotensive effects. Tomato (Solanum lycopersicum L.) is among the most widely cultivated and consumed vegetables in the world and contains higher levels of GABA than other major crops. Increasing these levels can further enhance the blood pressure-lowering function of tomato fruit. Glutamate decarboxylase (GAD) is a key enzyme in GABA biosynthesis; it has a C-terminal autoinhibitory domain that regulates enzymatic function, and deleting this domain increases GAD activity. The tomato genome has five GAD genes (SlGAD1-5), of which two (SlGAD2 and SlGAD3) are expressed during tomato fruit development. To increase GABA content in tomato, we deleted the autoinhibitory domain of SlGAD2 and SlGAD3 using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas)9 technology. Introducing a stop codon immediately before the autoinhibitory domain increased GABA accumulation by 7 to 15 fold while having variable effects on plant and fruit size and yield. This is the first study describing the application of the CRISPR/Cas9 system to increase GABA content in tomato fruits. Our findings provide a basis for the improvement of other types of crop by CRISPR/Cas9-based genetic modification.
巻・号 7(1)
ページ 7057
公開日 2017-8-1
DOI 10.1038/s41598-017-06400-y
PII 10.1038/s41598-017-06400-y
PMID 28765632
PMC PMC5539196
MeSH CRISPR-Associated Protein 9 Clustered Regularly Interspaced Short Palindromic Repeats Fruit / enzymology Fruit / genetics Fruit / metabolism Gene Editing Glutamate Decarboxylase / genetics Glutamate Decarboxylase / metabolism* Metabolic Engineering / methods* Metabolic Networks and Pathways / genetics Mutagenesis* Sequence Deletion* Solanum lycopersicum / enzymology Solanum lycopersicum / genetics Solanum lycopersicum / metabolism* gamma-Aminobutyric Acid / metabolism*
IF 3.998
引用数 33
リソース情報
トマト TOMJPF00001