RRC ID 49310
Author Abdelkareem A, Thagun C, Nakayasu M, Mizutani M, Hashimoto T, Shoji T.
Title Jasmonate-induced biosynthesis of steroidal glycoalkaloids depends on COI1 proteins in tomato.
Journal Biochem. Biophys. Res. Commun.
Abstract In tomato, perception of jasmonates by a receptor complex, which includes the F-box protein CORONATINE INSENSITIVE 1 (COI1), elicits biosynthesis of defensive steroidal glycoalkaloids (SGAs) via a jasmonate-responsive ERF transcription factor, JRE4/GAME9. Although JRE4 is upregulated by jasmonate and induces the expression of many metabolic genes involved in SGA biosynthesis, it is not known whether JRE4 alone is sufficient for increased SGA biosynthesis upon activation of jasmonate signaling. Here, we show that application of methyl jasmonate induces the expression of JRE4 and SGA biosynthesis genes in leaves and hairy roots of wild-type tomato, but not in jasmonic acid insensitive 1 (jai1), a loss-of-function mutant allele of the tomato COI1 gene. Induced overexpression of JRE4 increased the expression of SGA biosynthesis genes in transgenic hairy roots of both wild-type tomato and the jai1 mutant, suggesting that JRE4 is the primary transcription factor that functions downstream of the jasmonate signaling pathway.
Volume 489(2)
Pages 206-210
Published 2017-7-22
DOI 10.1016/j.bbrc.2017.05.132
PII S0006-291X(17)31025-2
PMID 28554842
MeSH Alkaloids / biosynthesis* Alkaloids / chemistry Cyclopentanes / administration & dosage Cyclopentanes / pharmacology* Lycopersicon esculentum / drug effects* Lycopersicon esculentum / metabolism* Oxylipins / administration & dosage Oxylipins / pharmacology* Phytosterols / biosynthesis* Phytosterols / chemistry Plant Proteins / genetics Plant Proteins / metabolism*
IF 2.559
Times Cited 0
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY BIOPHYSICS
Resource
Tomato TOMJPF00001