RRC ID 49312
Author Paupière MJ, Müller F, Li H, Rieu I, Tikunov YM, Visser RGF, Bovy AG.
Title Untargeted metabolomic analysis of tomato pollen development and heat stress response.
Journal Plant Reprod
Abstract KEY MESSAGE:Pollen development metabolomics. Developing pollen is among the plant structures most sensitive to high temperatures, and a decrease in pollen viability is often associated with an alteration of metabolite content. Most of the metabolic studies of pollen have focused on a specific group of compounds, which limits the identification of physiologically important metabolites. To get a better insight into pollen development and the pollen heat stress response, we used a liquid chromatography-mass spectrometry platform to detect secondary metabolites in pollen of tomato (Solanum lycopersicum L.) at three developmental stages under control conditions and after a short heat stress at 38 °C. Under control conditions, the young microspores accumulated a large amount of alkaloids and polyamines, whereas the mature pollen strongly accumulated flavonoids. The heat stress treatment led to accumulation of flavonoids in the microspore. The biological role of the detected metabolites is discussed. This study provides the first untargeted metabolomic analysis of developing pollen under a changing environment that can serve as reference for further studies.
Volume 30(2)
Pages 81-94
Published 2017-6
DOI 10.1007/s00497-017-0301-6
PII 10.1007/s00497-017-0301-6
PMID 28508929
PMC PMC5486769
MeSH Alkaloids / metabolism Chromatography, Gas Chromatography, Liquid Flavonoids / metabolism Heat-Shock Response* Lycopersicon esculentum / cytology Lycopersicon esculentum / metabolism* Metabolomics Pollen / growth & development* Pollen / metabolism Polyamines / metabolism Secondary Metabolism
IF 2.707
Times Cited 0
WOS Category PLANT SCIENCES REPRODUCTIVE BIOLOGY
Resource
Tomato TOMJPF00001