| RRC ID |
49317
|
| Author |
Yoshida T, Hamano A, Ueda A, Takeuchi H, Yamaoka S.
|
| Title |
Human SMOOTHENED inhibits human immunodeficiency virus type 1 infection.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Human SMOOTHENED (SMO) was identified by expression cloning as a new host factor that inhibits HIV-1 infection. Forced expression of SMO inhibited HIV-1 replication and infection with a single-round lentiviral vector, but not infection with a murine leukemia virus-based retroviral vector in human MT-4 T cells. Quantitative PCR analyses revealed that stable expression of SMO impaired formation of the integrated form of lentiviral DNA, but did not interrupt reverse transcription. This inhibition was evident in MT-4 and HUT102 human T cell lines expressing low levels of SMO mRNA, but not in SupT1 or Jurkat T cell lines expressing higher levels of SMO mRNA. Depletion of SMO mRNA in Jurkat cells facilitated HIV-1 vector infection, suggesting that endogenous SMO plays a role in limiting lentiviral infection. These results suggest that SMO inhibits HIV-1 replication after completion of reverse transcription but before integration.
|
| Volume |
493(1)
|
| Pages |
132-138
|
| Published |
2017-11-4
|
| DOI |
10.1016/j.bbrc.2017.09.063
|
| PII |
S0006-291X(17)31823-5
|
| PMID |
28917838
|
| MeSH |
HIV Infections / immunology*
HIV-1 / immunology*
Humans
Jurkat Cells
Smoothened Receptor / immunology*
Virus Internalization*
Virus Replication / immunology*
|
| IF |
2.985
|
| Times Cited |
1
|
| Resource |
| DNA material |
pCAG-HIVgp IN-D64V (RDB08256)
CSII-CMV-MCS-IRES2-Bsd (RDB04385). |
