論文 - 詳細
| RRC ID | 49317 |
|---|---|
| 著者 | Yoshida T, Hamano A, Ueda A, Takeuchi H, Yamaoka S. |
| タイトル | Human SMOOTHENED inhibits human immunodeficiency virus type 1 infection. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Human SMOOTHENED (SMO) was identified by expression cloning as a new host factor that inhibits HIV-1 infection. Forced expression of SMO inhibited HIV-1 replication and infection with a single-round lentiviral vector, but not infection with a murine leukemia virus-based retroviral vector in human MT-4 T cells. Quantitative PCR analyses revealed that stable expression of SMO impaired formation of the integrated form of lentiviral DNA, but did not interrupt reverse transcription. This inhibition was evident in MT-4 and HUT102 human T cell lines expressing low levels of SMO mRNA, but not in SupT1 or Jurkat T cell lines expressing higher levels of SMO mRNA. Depletion of SMO mRNA in Jurkat cells facilitated HIV-1 vector infection, suggesting that endogenous SMO plays a role in limiting lentiviral infection. These results suggest that SMO inhibits HIV-1 replication after completion of reverse transcription but before integration. |
| 巻・号 | 493(1) |
| ページ | 132-138 |
| 公開日 | 2017-11-4 |
| DOI | 10.1016/j.bbrc.2017.09.063 |
| PII | S0006-291X(17)31823-5 |
| PMID | 28917838 |
| MeSH | HIV Infections / immunology* HIV-1 / immunology* Humans Jurkat Cells Smoothened Receptor / immunology* Virus Internalization* Virus Replication / immunology* |
| IF | 2.985 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCAG-HIVgp IN-D64V (RDB08256) CSII-CMV-MCS-IRES2-Bsd (RDB04385). |