RRC ID 49322
著者 Tokuhiro SI, Tokuoka M, Kobayashi K, Kubo A, Oda-Ishii I, Satou Y.
タイトル Differential gene expression along the animal-vegetal axis in the ascidian embryo is maintained by a dual functional protein Foxd.
ジャーナル PLoS Genet.
Abstract In many animal embryos, a specific gene expression pattern is established along the animal-vegetal axis soon after zygotic transcription begins. In the embryo of the ascidian Ciona intestinalis, soon after the division that separates animal and vegetal hemispheres into distinct blastomeres, maternal Gata.a and β-catenin activate specific genes in the animal and vegetal blastomeres, respectively. On the basis of these initial distinct gene expression patterns, gene regulatory networks promote animal cells to become ectodermal tissues and vegetal cells to become endomesodermal tissues and a part of the nerve cord. In the vegetal hemisphere, β-catenin directly activates Foxd, an essential transcription factor gene for specifying endomesodermal fates. In the present study, we found that Foxd also represses the expression of genes that are activated specifically in the animal hemisphere, including Dmrt1, Prdm1-r.a (Bz1), Prdm1-r.b (Bz2), and Otx. A reporter assay showed that Dmrt1 expression was directly repressed by Foxd, and a chromatin immunoprecipitation assay showed that Foxd was bound to the upstream regions of Dmrt1, Prdm1-r.a, Prdm1-r.b, and Otx. Thus, Foxd has a dual function of activating specific gene expression in the vegetal hemisphere and of repressing the expression of genes that are normally expressed in the animal hemisphere. This dual function stabilizes the initial patterning along the animal-vegetal axis by β-catenin and Gata.a.
巻・号 13(5)
ページ e1006741
公開日 2017-5
DOI 10.1371/journal.pgen.1006741
PMID 28520732
PMC PMC5453608
MeSH Animals Body Patterning Ciona intestinalis / embryology Ciona intestinalis / genetics* Ciona intestinalis / metabolism Forkhead Transcription Factors / genetics Forkhead Transcription Factors / metabolism* Gene Expression Regulation, Developmental* Otx Transcription Factors / genetics Otx Transcription Factors / metabolism Transcription Factors / genetics Transcription Factors / metabolism Transcriptional Activation
カタユウレイボヤ・(ニッポンウミシダ) Wild C. int