RRC ID 4933
著者 Kurihara S, Oda S, Tsuboi Y, Kim HG, Oshida M, Kumagai H, Suzuki H.
タイトル gamma-Glutamylputrescine synthetase in the putrescine utilization pathway of Escherichia coli K-12.
ジャーナル J Biol Chem
Abstract Glutamate-putrescine ligase (gamma-glutamylputrescine synthetase, PuuA, EC 6.3.1.11) catalyzes the gamma-glutamylation of putrescine, the first step in a novel putrescine utilization pathway involving gamma-glutamylated intermediates, the Puu pathway, in Escherichia coli. In this report, the character and physiological importance of PuuA are described. Purified non-tagged PuuA catalyzed the ATP-dependent gamma-glutamylation of putrescine. The K(m) values for glutamate, ATP, and putrescine are 2.07, 2.35, and 44.6 mm, respectively. There are two putrescine utilization pathways in E. coli: the Puu pathway and the pathway without gamma-glutamylation. Gene deletion experiments of puuA, however, indicated that the Puu pathway was more critical in utilizing putrescine as a sole carbon or nitrogen source. The transcription of puuA was induced by putrescine and in a puuR-deleted strain. The amino acid sequences of PuuA and glutamine synthetase (GS) show high similarity. The molecular weights of the monomers of the two enzymes are quite similar, and PuuA exists as a dodecamer as does GS. Moreover the two amino acid residues of E. coli GS that are important for the metal-catalyzed oxidation of the enzyme molecule involved in protein turnover are conserved in PuuA, and it was experimentally shown that the corresponding amino acid residues in PuuA were involved in the metal-catalyzed oxidation similarly to GS. It is suggested that the intracellular concentration of putrescine is optimized by PuuA transcriptionally and posttranslationally and that excess putrescine is converted to a nutrient source by the Puu pathway.
巻・号 283(29)
ページ 19981-90
公開日 2008-7-18
DOI 10.1074/jbc.M800133200
PII S0021-9258(20)59652-X
PMID 18495664
MeSH Amino Acid Sequence Ammonia / metabolism Catalytic Domain Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / chemistry Escherichia coli Proteins / genetics Escherichia coli Proteins / isolation & purification Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Ligases / chemistry Ligases / genetics Ligases / isolation & purification Ligases / metabolism* Molecular Sequence Data Multigene Family / genetics Mutagenesis, Site-Directed Putrescine / metabolism* Sequence Alignment Sequence Homology, Amino Acid Substrate Specificity Transaminases / metabolism Transcription, Genetic / genetics
IF 4.238
引用数 47
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) JD22571 JD24090 ME7986(MG1655) pACYC184 pBR322 pUC19