RRC ID 49516
著者 Khandelwal R, Sipani R, Govinda Rajan S, Kumar R, Joshi R.
タイトル Combinatorial action of Grainyhead, Extradenticle and Notch in regulating Hox mediated apoptosis in Drosophila larval CNS.
ジャーナル PLoS Genet.
Abstract Hox mediated neuroblast apoptosis is a prevalent way to pattern larval central nervous system (CNS) by different Hox genes, but the mechanism of this apoptosis is not understood. Our studies with Abdominal-A (Abd-A) mediated larval neuroblast (pNB) apoptosis suggests that AbdA, its cofactor Extradenticle (Exd), a helix-loop-helix transcription factor Grainyhead (Grh), and Notch signaling transcriptionally contribute to expression of RHG family of apoptotic genes. We find that Grh, AbdA, and Exd function together at multiple motifs on the apoptotic enhancer. In vivo mutagenesis of these motifs suggest that they are important for the maintenance of the activity of the enhancer rather than its initiation. We also find that Exd function is independent of its known partner homothorax in this apoptosis. We extend some of our findings to Deformed expressing region of sub-esophageal ganglia where pNBs undergo a similar Hox dependent apoptosis. We propose a mechanism where common players like Exd-Grh-Notch work with different Hox genes through region specific enhancers to pattern respective segments of larval central nervous system.
巻・号 13(10)
ページ e1007043
公開日 2017-10-13
DOI 10.1371/journal.pgen.1007043
PMID 29023471
PMC PMC5667929
MeSH Amino Acid Sequence Animals Apoptosis / genetics* Central Nervous System / growth & development* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Drosophila / genetics* Drosophila / growth & development Drosophila Proteins / genetics Drosophila Proteins / metabolism* Enhancer Elements, Genetic Female Gene Expression Regulation, Developmental Genes, Homeobox Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* Larva / genetics Larva / growth & development Male Nuclear Proteins / genetics Nuclear Proteins / metabolism Receptors, Notch / genetics Receptors, Notch / metabolism* Transcription Factors / genetics Transcription Factors / metabolism*
ショウジョウバエ 17117R-4