論文 - 詳細
| RRC ID | 49531 |
|---|---|
| 著者 | Fujii Y, Maekawa S, Morita M. |
| タイトル | Astrocyte calcium waves propagate proximally by gap junction and distally by extracellular diffusion of ATP released from volume-regulated anion channels. |
| ジャーナル | Sci Rep |
| Abstract |
Wave-like propagation of [Ca2+]i increases is a remarkable intercellular communication characteristic in astrocyte networks, intercalating neural circuits and vasculature. Mechanically-induced [Ca2+]i increases and their subsequent propagation to neighboring astrocytes in culture is a classical model of astrocyte calcium wave and is known to be mediated by gap junction and extracellular ATP, but the role of each pathway remains unclear. Pharmacologic analysis of time-dependent distribution of [Ca2+]i revealed three distinct [Ca2+]i increases, the largest being in stimulated cells independent of extracellular Ca2+ and inositol 1,4,5-trisphosphate-induced Ca2+ release. In addition, persistent [Ca2+]i increases were found to propagate rapidly via gap junctions in the proximal region, and transient [Ca2+]i increases were found to propagate slowly via extracellular ATP in the distal region. Simultaneous imaging of astrocyte [Ca2+]i and extracellular ATP, the latter of which was measured by an ATP sniffing cell, revealed that ATP was released within the proximal region by volume-regulated anion channel in a [Ca2+]i independent manner. This detailed analysis of a classical model is the first to address the different contributions of two major pathways of calcium waves, gap junctions and extracellular ATP. |
| 巻・号 | 7(1) |
| ページ | 13115 |
| 公開日 | 2017-10-13 |
| DOI | 10.1038/s41598-017-13243-0 |
| PII | 10.1038/s41598-017-13243-0 |
| PMID | 29030562 |
| PMC | PMC5640625 |
| MeSH | Adenosine Triphosphate / metabolism* Animals Astrocytes / metabolism* Fluorescent Antibody Technique Gap Junctions / metabolism* Ion Channels / metabolism* Lipopolysaccharides / metabolism* Male Rats Receptors, Lipoprotein / metabolism |
| IF | 3.998 |
| 引用数 | 25 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pN1-G-CaMP2 (RDB06750). |