Reference - Detail
RRC ID | 49567 |
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Author | Kidou S, Tsukamoto S, Kobayashi S, Ejiri S. |
Title | Isolation and characterization of a rice cDNA encoding the gamma-subunit of translation elongation factor 1B (eEF1Bgamma). |
Journal | FEBS Lett |
Abstract |
We isolated a rice cDNA clone (refg) encoding the gamma-subunit of translation elongation factor 1B (eEF-1B gamma; the old designation was EF-1 gamma). The refg encodes an open reading frame of 419 amino acids which shows a similarity to the equivalent sequences from animals and yeast. Complex formation analysis, which showed the recombinant protein of refg (His-eEF1B gamma) and formed a complex with GST-eEF-1Bbeta, indicated that the refg encodes rice eEF1B gamma of the eEF1B alphabeta gamma complex. Expression analysis showed that refg mRNA is very abundant in suspension-cultured cells during the exponential phase of growth. A DNA blot analysis indicated that refg is located at a single locus in the rice genome. |
Volume | 434(3) |
Pages | 382-6 |
Published | 1998-9-4 |
DOI | 10.1016/s0014-5793(98)01014-x |
PII | S001457939801014X |
PMID | 9742959 |
MeSH | Amino Acid Sequence Animals Base Sequence Blotting, Southern Cloning, Molecular DNA, Complementary Humans Molecular Sequence Data Oryza / genetics* Peptide Elongation Factor 1 Peptide Elongation Factors / genetics Peptide Elongation Factors / isolation & purification* Sequence Homology, Amino Acid |
IF | 3.057 |
Times Cited | 7 |
Resource | |
DNA material | refg (RDB02083) |