RRC ID 49567
Author Kidou S, Tsukamoto S, Kobayashi S, Ejiri S.
Title Isolation and characterization of a rice cDNA encoding the gamma-subunit of translation elongation factor 1B (eEF1Bgamma).
Journal FEBS Lett
Abstract We isolated a rice cDNA clone (refg) encoding the gamma-subunit of translation elongation factor 1B (eEF-1B gamma; the old designation was EF-1 gamma). The refg encodes an open reading frame of 419 amino acids which shows a similarity to the equivalent sequences from animals and yeast. Complex formation analysis, which showed the recombinant protein of refg (His-eEF1B gamma) and formed a complex with GST-eEF-1Bbeta, indicated that the refg encodes rice eEF1B gamma of the eEF1B alphabeta gamma complex. Expression analysis showed that refg mRNA is very abundant in suspension-cultured cells during the exponential phase of growth. A DNA blot analysis indicated that refg is located at a single locus in the rice genome.
Volume 434(3)
Pages 382-6
Published 1998-9-4
DOI 10.1016/s0014-5793(98)01014-x
PII S001457939801014X
PMID 9742959
MeSH Amino Acid Sequence Animals Base Sequence Blotting, Southern Cloning, Molecular DNA, Complementary Humans Molecular Sequence Data Oryza / genetics* Peptide Elongation Factor 1 Peptide Elongation Factors / genetics Peptide Elongation Factors / isolation & purification* Sequence Homology, Amino Acid
IF 3.057
Times Cited 7
DNA material refg (RDB02083)