Reference - Detail
|Author||Kidou S, Tsukamoto S, Kobayashi S, Ejiri S.|
|Title||Isolation and characterization of a rice cDNA encoding the gamma-subunit of translation elongation factor 1B (eEF1Bgamma).|
We isolated a rice cDNA clone (refg) encoding the gamma-subunit of translation elongation factor 1B (eEF-1B gamma; the old designation was EF-1 gamma). The refg encodes an open reading frame of 419 amino acids which shows a similarity to the equivalent sequences from animals and yeast. Complex formation analysis, which showed the recombinant protein of refg (His-eEF1B gamma) and formed a complex with GST-eEF-1Bbeta, indicated that the refg encodes rice eEF1B gamma of the eEF1B alphabeta gamma complex. Expression analysis showed that refg mRNA is very abundant in suspension-cultured cells during the exponential phase of growth. A DNA blot analysis indicated that refg is located at a single locus in the rice genome.
|MeSH||Amino Acid Sequence Animals Base Sequence Blotting, Southern Cloning, Molecular DNA, Complementary Humans Molecular Sequence Data Oryza / genetics* Peptide Elongation Factor 1 Peptide Elongation Factors / genetics Peptide Elongation Factors / isolation & purification* Sequence Homology, Amino Acid|
|DNA material||refg (RDB02083)|