論文 - 詳細
RRC ID | 49567 |
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著者 | Kidou S, Tsukamoto S, Kobayashi S, Ejiri S. |
タイトル | Isolation and characterization of a rice cDNA encoding the gamma-subunit of translation elongation factor 1B (eEF1Bgamma). |
ジャーナル | FEBS Lett |
Abstract |
We isolated a rice cDNA clone (refg) encoding the gamma-subunit of translation elongation factor 1B (eEF-1B gamma; the old designation was EF-1 gamma). The refg encodes an open reading frame of 419 amino acids which shows a similarity to the equivalent sequences from animals and yeast. Complex formation analysis, which showed the recombinant protein of refg (His-eEF1B gamma) and formed a complex with GST-eEF-1Bbeta, indicated that the refg encodes rice eEF1B gamma of the eEF1B alphabeta gamma complex. Expression analysis showed that refg mRNA is very abundant in suspension-cultured cells during the exponential phase of growth. A DNA blot analysis indicated that refg is located at a single locus in the rice genome. |
巻・号 | 434(3) |
ページ | 382-6 |
公開日 | 1998-9-4 |
DOI | 10.1016/s0014-5793(98)01014-x |
PII | S001457939801014X |
PMID | 9742959 |
MeSH | Amino Acid Sequence Animals Base Sequence Blotting, Southern Cloning, Molecular DNA, Complementary Humans Molecular Sequence Data Oryza / genetics* Peptide Elongation Factor 1 Peptide Elongation Factors / genetics Peptide Elongation Factors / isolation & purification* Sequence Homology, Amino Acid |
IF | 3.057 |
引用数 | 7 |
リソース情報 | |
遺伝子材料 | refg (RDB02083) |