Reference - Detail
| RRC ID | 49570 |
|---|---|
| Author | Saito K, Tatsuguchi K, Murakoshi I, Hirano H. |
| Title | cDNA cloning and expression of cysteine synthase B localized in chloroplasts of Spinacia oleracea. |
| Journal | FEBS Lett |
| Abstract |
The cDNA clones for cysteine synthase B, which is localized in chloroplasts of Spinacia oleracea L., were isolated by screening a library with synthetic oligonucleotides encoding a partial peptide sequence of the purified protein. Nucleotide sequence analysis revealed an open reading frame encoding a polypeptide of 383 amino acids containing a putative transit peptide of 52 amino acids. A bacterial expression vector of the cDNA clone could genetically complement an Escherichia coli auxotroph lacking cysteine synthase and could produce the functionally active and immuno-reactive cysteine synthase in E. coli. RNA blot hybridization suggested that the transcripts were primarily accumulated in leaves of spinach. |
| Volume | 324(3) |
| Pages | 247-52 |
| Published | 1993-6-21 |
| DOI | 10.1016/0014-5793(93)80127-g |
| PII | 0014-5793(93)80127-G |
| PMID | 8405359 |
| MeSH | Amino Acid Sequence Base Sequence Chloroplasts / enzymology* Cloning, Molecular Consensus Sequence DNA, Complementary / genetics Gene Expression Genes, Plant Genetic Complementation Test Lyases / genetics* Molecular Sequence Data Oligonucleotide Probes / chemistry Plant Proteins / genetics Plant Proteins / metabolism Sequence Alignment Sequence Homology, Amino Acid Vegetables / genetics |
| IF | 3.057 |
| Times Cited | 53 |
| Resource | |
| DNA material | PCSB II (RDB01537) |