RRC ID 49583
Author Ishikawa R, Iwata M, Taniko K, Monden G, Miyazaki N, Orn C, Tsujimura Y, Yoshida S, Ma JF, Ishii T.
Title Detection of quantitative trait loci controlling grain zinc concentration using Australian wild rice, Oryza meridionalis, a potential genetic resource for biofortification of rice.
Journal PLoS One
Abstract Zinc (Zn) is one of the essential mineral elements for both plants and humans. Zn deficiency in human is one of the major causes of hidden hunger, a serious health problem observed in many developing countries. Therefore, increasing Zn concentration in edible part is an important issue for improving human Zn nutrition. Here, we found that an Australian wild rice O. meridionalis showed higher grain Zn concentrations compared with cultivated and other wild rice species. The quantitative trait loci (QTL) analysis was then performed to identify the genomic regions controlling grain Zn levels using backcross recombinant inbred lines derived from O. sativa 'Nipponbare' and O. meridionalis W1627. Four QTLs responsible for high grain Zn were detected on chromosomes 2, 9, and 10. The QTL on the chromosome 9 (named qGZn9), which showed the largest effect on grain Zn concentration was confirmed with the introgression line, which had a W1627 chromosomal segment covering the qGZn9 region in the genetic background of O. sativa 'Nipponbare'. Fine mapping of this QTL resulted in identification of two tightly linked loci, qGZn9a and qGZn9b. The candidate regions of qGZn9a and qGZn9b were estimated to be 190 and 950 kb, respectively. Furthermore, we also found that plants having a wild chromosomal segment covering qGZn9a, but not qGZn9b, is associated with fertility reduction. qGZn9b, therefore, provides a valuable allele for breeding rice with high Zn in the grains.
Volume 12(10)
Pages e0187224
Published 2017-1-1
DOI 10.1371/journal.pone.0187224
PII PONE-D-17-30655
PMID 29077764
PMC PMC5659790
MeSH Australia Genes, Plant Oryza / genetics* Oryza / metabolism Quantitative Trait Loci* Zinc / metabolism*
IF 2.776
Times Cited 4
Rice W0630 W1169 W1152 W1627 W1298 W1561 W1629 W1631 W1635 W2069 W2080 W2081 W2103 W2116