| RRC ID |
4960
|
| Author |
Fukiya S, Arata M, Kawashima H, Yoshida D, Kaneko M, Minamida K, Watanabe J, Ogura Y, Uchida K, Itoh K, Wada M, Ito S, Yokota A.
|
| Title |
Conversion of cholic acid and chenodeoxycholic acid into their 7-oxo derivatives by Bacteroides intestinalis AM-1 isolated from human feces.
|
| Journal |
FEMS Microbiol Lett
|
| Abstract |
Secondary bile acid-producing bacteria were isolated from human feces to improve our appreciation of the functional diversity and redundancy of the intestinal microbiota. In total, 619 bacterial colonies were isolated using a nutrient-poor agar medium and the level of secondary bile acid formation was examined in each by a liquid culture, followed by thin-layer chromatography. Of five strains analyzed by 16S rRNA gene sequencing and biochemical testing, one was identified as Bacteroides intestinalis AM-1, which was not previously recognized as a secondary bile-acid producer. GC-MS revealed that B. intestinalis AM-1 converts cholic acid (CA) and chenodeoxycholic acid into their 7-oxo derivatives, 7-oxo-deoxycholic acid (7-oxo-DCA) and 7-oxo-lithocholic acid, respectively. Thus, B. intestinalis AM-1 possesses 7alpha-hydroxysteroid dehydrogenase (7alpha-HSDH) activity. In liquid culture, B. intestinalis AM-1 showed a relatively higher productivity of 7-oxo-DCA than Escherichia coli HB101 and Bacteroides fragilis JCM11019(T), which are known to possess 7alpha-HSDH activity. The level of 7alpha-HSDH activity was higher in B. intestinalis AM-1 than in the other two strains under the conditions tested. The 7alpha-HSDH activity in each of the three strains is not induced by CA; instead, it is regulated in a growth phase-dependent manner.
|
| Volume |
293(2)
|
| Pages |
263-70
|
| Published |
2009-4-1
|
| DOI |
10.1111/j.1574-6968.2009.01531.x
|
| PII |
FML1531
|
| PMID |
19243441
|
| MeSH |
Adult
Bacterial Typing Techniques
Bacteroides / classification
Bacteroides / growth & development
Bacteroides / isolation & purification
Bacteroides / metabolism*
Bacteroides fragilis / metabolism
Biotransformation
Chenodeoxycholic Acid / metabolism*
Cholic Acid / metabolism*
Chromatography, Thin Layer
Culture Media / chemistry
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Deoxycholic Acid / analogs & derivatives
Deoxycholic Acid / metabolism
Escherichia coli / metabolism
Feces / microbiology*
Female
Humans
Lithocholic Acid / analogs & derivatives
Lithocholic Acid / metabolism
Male
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Young Adult
|
| IF |
1.987
|
| Times Cited |
45
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| Prokaryotes E. coli |
ME8568 (HB101) |
| General Microbes |
JCM 11019
JCM 13265 |
