RRC ID 49614
Author Ohta A, Tsunoda Y, Tamura Y, Iino K, Nishimura N, Nishihara H, Takanashi H, Yoshida S, Kato T, Kato Y.
Title Construction and expression of vectors encoding biologically active rodent gonadotropins.
Journal J Reprod Dev
Abstract The gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), are important hormones in vertebrate reproduction. The isolation of gonadotropins from the pituitary gland is sub-optimal, as the cross-contamination of one hormone with another is common and often results in the variation in the measured activity of LH and FSH. The production of recombinant hormones is, therefore, a viable approach to solve this problem. This study aimed to express recombinant rat, mouse, and mastomys FSH and LH in Chinese hamster ovary (CHO) cells. Their common α-subunits along with their hormone-specific β-subunits were encoded in a single mammalian expression vector. FSH from all three species was expressed, whereas expression was achieved only for the mouse LH. Immunohistochemistry for rat alpha subunit of glycoprotein hormone (αGSU) and LHβ and FSHβ subunits confirmed the production of the dimeric hormone in CHO cells. The recombinant rodent gonadotropins were confirmed to be biologically active; estradiol production was increased by recombinant FSH in granulosa cells, while recombinant LH increased testosterone production in Leydig cells.
Volume 63(6)
Pages 605-609
Published 2017-12-15
DOI 10.1262/jrd.2017-091
PMID 29033405
PMC PMC5735272
MeSH Animals CHO Cells Cricetulus Follicle Stimulating Hormone / biosynthesis* Follicle Stimulating Hormone / genetics Genetic Vectors* Luteinizing Hormone / biosynthesis* Luteinizing Hormone / genetics Male Mice, Inbred C57BL Murinae Rats, Wistar Recombinant Proteins / biosynthesis
IF 1.635
Times Cited 0
DNA material pCX-puro-ratLH (RDB15146) pCX-puro-ratFSH (RDB15147) pCX-PURO-mouLH (RDB15148) pCX-PURO-mouFSH (RDB15149) pCX-PURO-masLH (RDB15150) pCX-PURO-masFSH (RDB15151).
Human and Animal Cells CHO-K1(RCB0285)