Reference - Detail
| RRC ID | 49628 |
|---|---|
| Author | Tsutsui H, Karasawa S, Okamura Y, Miyawaki A. |
| Title | Improving membrane voltage measurements using FRET with new fluorescent proteins. |
| Journal | Nat Methods |
| Abstract |
We used two new coral fluorescent proteins as fluorescence resonance energy transfer (FRET) donor and acceptor to develop a voltage sensor, named Mermaid, that displays approximately 40% changes in emission ratio per 100 mV, allowing for direct visualization of electrical activities in cultured excitable cells. Notably, Mermaid has fast on-off kinetics at warm (approximately 33 degrees C) temperatures and can report voltage spikes comparable to action potentials. |
| Volume | 5(8) |
| Pages | 683-5 |
| Published | 2008-8-1 |
| DOI | 10.1038/nmeth.1235 |
| PII | nmeth.1235 |
| PMID | 18622396 |
| MeSH | Cell Membrane* Fluorescence Resonance Energy Transfer / methods* Kinetics Luminescent Proteins / analysis* Luminescent Proteins / genetics Luminescent Proteins / metabolism Membrane Potentials* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism |
| IF | 30.822 |
| Times Cited | 192 |
| Resource | |
| DNA material | Mermaid (RDB15262) hmKO-K/pRSETB (RDB15314) CoralHue™ monomeric Umikinoko-Green 1 (pmUkG1-S1) (RDB18306) CoralHue™ humanized monomeric Umikinoko-Green 1 (phmUkG1-S1) (RDB18307) CoralHue™ humanized monomeric Umikinoko-Green 1 (phmUkG1-MC1) (RDB18308) CoralHue™ humanized monomeric Umikinoko-Green 1 (phmUkG1-MN1) (RDB18309) |