論文 - 詳細
| RRC ID | 49660 |
|---|---|
| 著者 | Kozawa S, Akanuma T, Sato T, Sato YD, Ikeda K, Sato TN. |
| タイトル | Real-time prediction of cell division timing in developing zebrafish embryo. |
| ジャーナル | Sci Rep |
| Abstract |
Combination of live-imaging and live-manipulation of developing embryos in vivo provides a useful tool to study developmental processes. Identification and selection of target cells for an in vivo live-manipulation are generally performed by experience- and knowledge-based decision-making of the observer. Computer-assisted live-prediction method would be an additional approach to facilitate the identification and selection of the appropriate target cells. Herein we report such a method using developing zebrafish embryos. We choose V2 neural progenitor cells in developing zebrafish embryo as their successive shape changes can be visualized in real-time in vivo. We developed a relatively simple mathematical method of describing cellular geometry of V2 cells to predict cell division-timing based on their successively changing shapes in vivo. Using quantitatively measured 4D live-imaging data, features of V2 cell-shape at each time point prior to division were extracted and a statistical model capturing the successive changes of the V2 cell-shape was developed. By applying sequential Bayesian inference method to the model, we successfully predicted division-timing of randomly selected individual V2 cells while the cell behavior was being live-imaged. This system could assist pre-selecting target cells desirable for real-time manipulation-thus, presenting a new opportunity for in vivo experimental systems. |
| 巻・号 | 6 |
| ページ | 32962 |
| 公開日 | 2016-9-6 |
| DOI | 10.1038/srep32962 |
| PII | srep32962 |
| PMID | 27597656 |
| PMC | PMC5011986 |
| MeSH | Animals Bayes Theorem Cell Division* Cell Line Embryonic Development Microscopy, Confocal Neural Stem Cells / cytology* Zebrafish / embryology* |
| IF | 3.998 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(vsx1:EGFP) |