RRC ID 49676
Author Hernandez O, Papagiakoumou E, Tanese D, Fidelin K, Wyart C, Emiliani V.
Title Three-dimensional spatiotemporal focusing of holographic patterns.
Journal Nat Commun
Abstract Two-photon excitation with temporally focused pulses can be combined with phase-modulation approaches, such as computer-generated holography and generalized phase contrast, to efficiently distribute light into two-dimensional, axially confined, user-defined shapes. Adding lens-phase modulations to 2D-phase holograms enables remote axial pattern displacement as well as simultaneous pattern generation in multiple distinct planes. However, the axial confinement linearly degrades with lateral shape area in previous reports where axially shifted holographic shapes were not temporally focused. Here we report an optical system using two spatial light modulators to independently control transverse- and axial-target light distribution. This approach enables simultaneous axial translation of single or multiple spatiotemporally focused patterns across the sample volume while achieving the axial confinement of temporal focusing. We use the system's capability to photoconvert tens of Kaede-expressing neurons with single-cell resolution in live zebrafish larvae.
Volume 7
Pages 11928
Published 2016-6-16
DOI 10.1038/ncomms11928
PII ncomms11928
PMID 27306044
PMC PMC4912686
MeSH Animals Holography / instrumentation Holography / methods* Image Processing, Computer-Assisted / methods Imaging, Three-Dimensional / methods* Larva / growth & development Larva / ultrastructure Light Microscopy, Phase-Contrast / instrumentation Microscopy, Phase-Contrast / methods* Neurons / ultrastructure* Optical Devices* Zebrafish / growth & development
IF 12.353
Resource
Zebrafish Tg(huC:GVP)