RRC ID 49682
Author Steventon B, Duarte F, Lagadec R, Mazan S, Nicolas JF, Hirsinger E.
Title Species-specific contribution of volumetric growth and tissue convergence to posterior body elongation in vertebrates.
Journal Development
Abstract Posterior body elongation is a widespread mechanism propelling the generation of the metazoan body plan. The posterior growth model predicts that a posterior growth zone generates sufficient tissue volume to elongate the posterior body. However, there are energy supply-related differences between vertebrates in the degree to which growth occurs concomitantly with embryogenesis. By applying a multi-scalar morphometric analysis in zebrafish embryos, we show that posterior body elongation is generated by an influx of cells from lateral regions, by convergence-extension of cells as they exit the tailbud, and finally by a late volumetric growth in the spinal cord and notochord. Importantly, the unsegmented region does not generate additional tissue volume. Fibroblast growth factor inhibition blocks tissue convergence rather than volumetric growth, showing that a conserved molecular mechanism can control convergent morphogenesis through different cell behaviours. Finally, via a comparative morphometric analysis in lamprey, dogfish, zebrafish and mouse, we propose that elongation via posterior volumetric growth is linked to increased energy supply and is associated with an overall increase in volumetric growth and elongation.
Volume 143(10)
Pages 1732-41
Published 2016-5-15
DOI 10.1242/dev.126375
PII dev.126375
PMID 26989170
MeSH Animals Body Patterning* Cell Movement Cell Proliferation Dogfish / embryology Fibroblast Growth Factors / metabolism Lampreys / embryology Mice Notochord / embryology Organogenesis* Signal Transduction Species Specificity Spinal Cord / embryology Tail Vertebrates / embryology* Zebrafish / embryology
IF 5.763
Times Cited 26
Resource
Zebrafish Tg(EF1α:mAG-zGem(1/100))