論文 - 詳細
| RRC ID | 49718 |
|---|---|
| 著者 | Ichise H, Nagano S, Maeda T, Miyazaki M, Miyazaki Y, Kojima H, Yawata N, Yawata M, Tanaka H, Saji H, Masuda K, Kawamoto H. |
| タイトル | NK Cell Alloreactivity against KIR-Ligand-Mismatched HLA-Haploidentical Tissue Derived from HLA Haplotype-Homozygous iPSCs. |
| ジャーナル | Stem Cell Reports |
| Abstract |
HLA haplotype-homozygous (HLA-homo) induced pluripotent stem cells (iPSCs) are being prepared to be used for allogeneic transplantation of regenerated tissue into recipients carrying an identical haplotype in one of the alleles (HLA-hetero). However, it remains unaddressed whether natural killer (NK) cells respond to these regenerated cells. HLA-C allotypes, known to serve as major ligands for inhibitory receptors of NK cells, can be classified into group 1 (C1) and group 2 (C2), based on their binding specificities. We found that the T cells and vascular endothelial cells regenerated from HLA-homo-C1/C1 iPSCs were killed by specific NK cell subsets from a putative HLA-hetero-C1/C2 recipient. Such cytotoxicity was canceled when target cells were regenerated from iPSCs transduced with the C2 gene identical to the recipient. These results clarify that NK cells can kill regenerated cells by sensing the lack of HLA-C expression and further provide the basis for an approach to prevent such NK cell-mediated rejection responses. |
| 巻・号 | 9(3) |
| ページ | 853-867 |
| 公開日 | 2017-9-12 |
| DOI | 10.1016/j.stemcr.2017.07.020 |
| PII | S2213-6711(17)30330-2 |
| PMID | 28867344 |
| PMC | PMC5599245 |
| MeSH | Asian People Cytotoxicity, Immunologic HLA Antigens / metabolism* Haplotypes / genetics* Homozygote Humans Immune Tolerance Induced Pluripotent Stem Cells / metabolism* Killer Cells, Natural / metabolism* Ligands Lymphocyte Subsets / metabolism Receptors, KIR / metabolism* Regeneration T-Lymphocytes / metabolism Tissue Donors Transplantation, Homologous |
| IF | 6.032 |
| 引用数 | 24 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 31 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | Human HLA cDNA HLA-C*04:01:01 (RDB02886) HLA-C*15:02:01 (RDB03379) HLA-C*01:02 (RDB02883) CS-UbC-RfA-IRES2-Venus (RDB08255). |