RRC ID 4985
Author Rath D, Mangoli SH, Mahajan SK, Jawali N.
Title A novel mutation spatially remote from the G-domain in IF2 affects the cold stress adaptation of Escherichia coli.
Journal Res Microbiol
Abstract In this paper, we describe a new mutation, gicD1, that gives a cold-sensitive phenotype in bacterial cell growth. Complementation analysis showed gicD1 to be allelic to infB. We identify gicD1 to be a valine to isoleucine substitution in initiation factor-2 (IF2) of a residue that seems to be well conserved in eubacterial IF2 proteins. This mutation lies in a region distant from the G-domain to which all earlier reported cold-sensitive mutations cluster. We describe a novel phenotype of the mutant that is suppression of rpsL31-mediated streptomycin resistance in cold. We provide evidence that mutant IF2 specifically interacts with rpsL31 in cold, leading to a bacteriostatic effect on host cells.
Volume 160(8)
Pages 576-80
Published 2009-10-1
DOI 10.1016/j.resmic.2009.08.002
PII S0923-2508(09)00129-6
PMID 19716413
MeSH Amino Acid Substitution / genetics Anti-Bacterial Agents / pharmacology Cold Temperature* Drug Resistance, Bacterial Escherichia coli / genetics Escherichia coli / growth & development Escherichia coli / physiology* Escherichia coli Proteins Genetic Complementation Test Mutation, Missense* Prokaryotic Initiation Factor-2 / genetics Prokaryotic Initiation Factor-2 / physiology* Protein Interaction Mapping Ribosomal Proteins / genetics Streptomycin / pharmacology Stress, Physiological* Suppression, Genetic
IF 3.217
Times Cited 3
Prokaryotes E. coli ME8913(CAG12122) ME7986(MG1655) W3110 ME8848(CAG12072) ME8796(CAG12077)