RRC ID |
4985
|
Author |
Rath D, Mangoli SH, Mahajan SK, Jawali N.
|
Title |
A novel mutation spatially remote from the G-domain in IF2 affects the cold stress adaptation of Escherichia coli.
|
Journal |
Res Microbiol
|
Abstract |
In this paper, we describe a new mutation, gicD1, that gives a cold-sensitive phenotype in bacterial cell growth. Complementation analysis showed gicD1 to be allelic to infB. We identify gicD1 to be a valine to isoleucine substitution in initiation factor-2 (IF2) of a residue that seems to be well conserved in eubacterial IF2 proteins. This mutation lies in a region distant from the G-domain to which all earlier reported cold-sensitive mutations cluster. We describe a novel phenotype of the mutant that is suppression of rpsL31-mediated streptomycin resistance in cold. We provide evidence that mutant IF2 specifically interacts with rpsL31 in cold, leading to a bacteriostatic effect on host cells.
|
Volume |
160(8)
|
Pages |
576-80
|
Published |
2009-10-1
|
DOI |
10.1016/j.resmic.2009.08.002
|
PII |
S0923-2508(09)00129-6
|
PMID |
19716413
|
MeSH |
Amino Acid Substitution / genetics
Anti-Bacterial Agents / pharmacology
Cold Temperature*
Drug Resistance, Bacterial
Escherichia coli / genetics
Escherichia coli / growth & development
Escherichia coli / physiology*
Escherichia coli Proteins
Genetic Complementation Test
Mutation, Missense*
Prokaryotic Initiation Factor-2 / genetics
Prokaryotic Initiation Factor-2 / physiology*
Protein Interaction Mapping
Ribosomal Proteins / genetics
Streptomycin / pharmacology
Stress, Physiological*
Suppression, Genetic
|
IF |
3.217
|
Times Cited |
3
|
WOS Category
|
MICROBIOLOGY
|
Resource |
Prokaryotes E. coli |
ME8913(CAG12122)
ME7986(MG1655)
W3110
ME8848(CAG12072)
ME8796(CAG12077) |