論文 - 詳細
| RRC ID | 4988 |
|---|---|
| 著者 | Shimizu T, Ohta Y, Noda M. |
| タイトル | Shiga toxin 2 is specifically released from bacterial cells by two different mechanisms. |
| ジャーナル | Infect Immun |
| Abstract |
Shiga toxin 1 (Stx1) is located in the periplasmic fraction, while Stx2 is found in the extracellular fraction, suggesting that enterohemorrhagic Escherichia coli (EHEC) contains a specific Stx2 release system. Both stx(1) and stx(2) are found within the late operons of Stx-encoding phages. Stx2 production is greatly induced by mitomycin C, suggesting that stx(2) can transcribe from the late phage promoter of the Stx2-encoding phage. However, the Stx1 promoter adjacent to stx(1) is a dominant regulatory element in Stx1 production. With the deletion of phage lysis genes of the Stx2-encoding phage, Stx2 remains in the bacterial cells. Further, we demonstrate that the Stx2-encoding phage, but not the Stx1-encoding phage, is spontaneously induced at extremely low rates. These results indicate that spontaneously specific Stx2-encoding phage induction is involved in specific Stx2 release from bacterial cells. Furthermore, to examine whether another system for specific Stx2 release is present in EHEC, we analyze the stx-replaced mutants. As expected, Stx2 derived from the Stx1 promoter is located in both the extracellular and cell-associated fractions, while mutant Stx2 (B subunit, S31N) derived from the Stx1 promoter is found only in the cell-associated fraction. These results indicate that EHEC has another Stx2 release system that strictly recognizes the serine 31 residue of the B subunit. Overall, we present evidence that specific Stx2 release from bacterial cells is involved in both the Stx2-encoding phage induction system and another Stx2 release system. |
| 巻・号 | 77(7) |
| ページ | 2813-23 |
| 公開日 | 2009-7-1 |
| DOI | 10.1128/IAI.00060-09 |
| PII | IAI.00060-09 |
| PMID | 19380474 |
| PMC | PMC2708558 |
| MeSH | Animals Bacteriolysis* Coliphages / growth & development Enterohemorrhagic Escherichia coli / metabolism* Enterohemorrhagic Escherichia coli / virology* Genes, Viral Prophages / growth & development Protein Transport Rabbits Shiga Toxin 2 / metabolism* |
| IF | 3.201 |
| 引用数 | 52 |
| WOS 分野 | INFECTIOUS DISEASES IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pFT-A |