論文 - 詳細
| RRC ID | 4997 |
|---|---|
| 著者 | Iwasaki T, Ohmori D, Shimizu N, Kumasaka T. |
| タイトル | Crystallization and preliminary X-ray diffraction studies of the ISC-like [2Fe-2S] ferredoxin (FdxB) from Pseudomonas putida JCM 20004. |
| ジャーナル | Acta Crystallogr Sect F Struct Biol Cryst Commun |
| Abstract |
The iron-sulfur (Fe-S) cluster-biosynthesis (ISC) system of the gamma-proteobacterium Pseudomonas putida JCM 20004 contains a constitutively expressed vertebrate-type [2Fe-2S] ferredoxin, FdxB, which lacks the conserved free cysteine residue near the Fe-S cluster site that has been proposed to function in the catalysis of biological Fe-S cluster assembly in other bacterial homologues. Recombinant FdxB was heterologously overproduced in Escherichia coli, purified and crystallized in its oxidized form by the hanging-drop vapour-diffusion and streak-seeding methods using 1.6 M trisodium citrate dihydrate pH 6.5. The thin needle-shaped crystals diffract to 1.90 A resolution and belong to the hexagonal space group P6(1)22, with unit-cell parameters a = 87.58, c = 73.14 A. The asymmetric unit contains one protein molecule. |
| 巻・号 | 63(Pt 12) |
| ページ | 1014-6 |
| 公開日 | 2007-12-1 |
| DOI | 10.1107/S1744309107045757 |
| PII | S1744309107045757 |
| PMID | 18084081 |
| PMC | PMC2344098 |
| MeSH | Amino Acid Sequence Conserved Sequence Crystallization Ferredoxins / chemistry* Ferredoxins / genetics Ferredoxins / metabolism Molecular Sequence Data Multigene Family / genetics* Pseudomonas putida / chemistry* Pseudomonas putida / genetics Pseudomonas putida / metabolism Sequence Alignment X-Ray Diffraction |
| IF | 0.968 |
| 引用数 | 4 |
| WOS 分野 | CRYSTALLOGRAPHY BIOPHYSICS BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM20004 |