RRC ID 4997
Author Iwasaki T, Ohmori D, Shimizu N, Kumasaka T.
Title Crystallization and preliminary X-ray diffraction studies of the ISC-like [2Fe-2S] ferredoxin (FdxB) from Pseudomonas putida JCM 20004.
Journal Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.
Abstract The iron-sulfur (Fe-S) cluster-biosynthesis (ISC) system of the gamma-proteobacterium Pseudomonas putida JCM 20004 contains a constitutively expressed vertebrate-type [2Fe-2S] ferredoxin, FdxB, which lacks the conserved free cysteine residue near the Fe-S cluster site that has been proposed to function in the catalysis of biological Fe-S cluster assembly in other bacterial homologues. Recombinant FdxB was heterologously overproduced in Escherichia coli, purified and crystallized in its oxidized form by the hanging-drop vapour-diffusion and streak-seeding methods using 1.6 M trisodium citrate dihydrate pH 6.5. The thin needle-shaped crystals diffract to 1.90 A resolution and belong to the hexagonal space group P6(1)22, with unit-cell parameters a = 87.58, c = 73.14 A. The asymmetric unit contains one protein molecule.
Volume 63(Pt 12)
Pages 1014-6
Published 2007-12-1
DOI 10.1107/S1744309107045757
PII S1744309107045757
PMID 18084081
PMC PMC2344098
MeSH Amino Acid Sequence Conserved Sequence Crystallization Ferredoxins / chemistry* Ferredoxins / genetics Ferredoxins / metabolism Molecular Sequence Data Multigene Family / genetics* Pseudomonas putida / chemistry* Pseudomonas putida / genetics Pseudomonas putida / metabolism Sequence Alignment X-Ray Diffraction
IF 0.989
Times Cited 3
WOS Category CRYSTALLOGRAPHY BIOPHYSICS BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
General Microbes JCM20004