RRC ID 50002
Author Hirano N, Marukawa Y, Abe J, Hashiba S, Ichikawa M, Tanabe Y, Ito M, Nishii I, Tsuchikane Y, Sekimoto H.
Title A Receptor-Like Kinase, Related to Cell Wall Sensor of Higher Plants, is Required for Sexual Reproduction in the Unicellular Charophycean Alga, Closterium peracerosum-strigosum-littorale Complex.
Journal Plant Cell Physiol
Abstract Here, we cloned the CpRLK1 gene, which encodes a receptor-like protein kinase expressed during sexual reproduction, from the heterothallic Closterium peracerosum-strigosum-littorale complex, one of the closest unicellular alga to land plants. Mating-type plus (mt(+)) cells with knockdown of CpRLK1 showed reduced competence for sexual reproduction and formed an abnormally enlarged conjugation papilla after pairing with mt(-) cells. The knockdown cells were unable to release a naked gamete, which is indispensable for zygote formation. We suggest that the CpRLK1 protein is an ancient cell wall sensor that now functions to regulate osmotic pressure in the cell to allow proper gamete release.
Volume 56(7)
Pages 1456-62
Published 2015-7-1
DOI 10.1093/pcp/pcv065
PII pcv065
PMID 25941232
MeSH Algal Proteins / classification Algal Proteins / genetics* Algal Proteins / metabolism Amino Acid Sequence Cell Wall / genetics Cell Wall / metabolism Cloning, Molecular Closterium / genetics* Closterium / metabolism Closterium / physiology DNA, Complementary / chemistry DNA, Complementary / genetics Gene Knockdown Techniques Immunoblotting Microscopy, Confocal Molecular Sequence Data Osmotic Pressure / physiology Phylogeny Plants / genetics Plants / metabolism Protein Kinases / classification Protein Kinases / genetics* Protein Kinases / metabolism Reproduction / genetics Reproduction / physiology Sequence Analysis, DNA Sequence Homology, Amino Acid Time-Lapse Imaging / methods
IF 4.062
Times Cited 10
Algae NIES-67 NIES-68