RRC ID 50012
Author Sugimori S, Kumata Y, Kobayashi S.
Title Maternal Nanos-Dependent RNA Stabilization in the Primordial Germ Cells of Drosophila Embryos.
Journal Dev. Growth Differ.
Abstract Nanos (Nos) is an evolutionary conserved protein expressed in the germline of various animal species. In Drosophila, maternal Nos protein is essential for germline development. In the germline progenitors, or the primordial germ cells (PGCs), Nos binds to the 3' UTR of target mRNAs to repress their translation. In contrast to this prevailing role of Nos, here we report that the 3' UTR of CG32425 mRNA mediates Nos-dependent RNA stabilization in PGCs. We found that the level of mRNA expressed from a reporter gene fused to the CG32425 3' UTR was significantly reduced in PGCs lacking maternal Nos (nos PGCs) as compared with normal PGCs. By deleting the CG32425 3' UTR, we identified the region required for mRNA stabilization, which includes Nos-binding sites. In normal embryos, CG32425 mRNA was maternally supplied into PGCs and remained in this cell type during embryogenesis. However, as expected from our reporter assay, the levels of CG32425 mRNA and its protein product expressed in nos PGCs were lower than in normal PGCs. Thus, we propose that Nos protein has dual functions in translational repression and stabilization of specific RNAs to ensure proper germline development.
Volume 60(1)
Pages 63-75
Published 2018-1
DOI 10.1111/dgd.12414
PMID 29278271
MeSH 3' Untranslated Regions Animals Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / embryology* Drosophila melanogaster / genetics* Drosophila melanogaster / physiology Embryo, Nonmammalian / cytology Embryo, Nonmammalian / physiology Female Gene Expression Regulation, Developmental Germ Cells / cytology Germ Cells / physiology* Male Maternal Inheritance Protein Biosynthesis* RNA Stability* RNA, Messenger / genetics RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism*
IF 1.496