RRC ID 50013
Author Tomaru M, Ohsako T, Watanabe M, Juni N, Matsubayashi H, Sato H, Takahashi A, Yamamoto MT.
Title Severe Fertility Effects of sheepish Sperm Caused by Failure To Enter Female Sperm Storage Organs in Drosophila melanogaster.
Journal G3 (Bethesda)
Abstract In Drosophila, mature sperm are transferred from males to females during copulation, stored in the sperm storage organs of females, and then utilized for fertilization. Here, we report a gene named sheepish (shps) of Drosophila melanogaster that is essential for sperm storage in females. shps mutant males, although producing morphologically normal and motile sperm that are effectively transferred to females, produce very few offspring. Direct counts of sperm indicated that the primary defect was correlated to failure of shps sperm to migrate into the female sperm storage organs. Increased sperm motion parameters were seen in the control after transfer to females, whereas sperm from shps males have characteristics of the motion parameters different from the control. The few sperm that occasionally entered the female sperm storage organs showed no obvious defects in fertilization and early embryo development. The female postmating responses after copulation with shps males appeared normal, at least with respect to conformational changes of uterus, mating plug formation, and female remating rates. The shps gene encodes a protein with homology to amine oxidases, including as observed in mammals, with a transmembrane region at the C-terminal end. The shps mutation was characterized by a nonsense replacement in the third exon of CG13611, and shps was rescued by transformants of the wild-type copy of CG13611 Thus, shps may define a new class of gene responsible for sperm storage.
Volume 8(1)
Pages 149-160
Published 2018-1-4
DOI 10.1534/g3.117.300171
PII g3.117.300171
PMID 29158336
PMC PMC5765343
MeSH Animal Structures / cytology Animal Structures / metabolism Animals Clutch Size Copulation Drosophila Proteins / deficiency Drosophila Proteins / genetics* Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Female Fertility Fertilization / genetics* Gene Expression Genetic Complementation Test Male Oxidoreductases Acting on CH-NH Group Donors / deficiency Oxidoreductases Acting on CH-NH Group Donors / genetics* Sperm Count Sperm Motility Spermatozoa / metabolism* Spermatozoa / pathology
IF 2.63
Resource
Drosophila DGRC#101738 DGRC#105666 DGRC#106416 DGRC#109173 DGRC#118171 DGRC#118172 DGRC#118173 DGRC#118174