RRC ID 5002
著者 Fujita K, Ichimasa S, Zendo T, Koga S, Yoneyama F, Nakayama J, Sonomoto K.
タイトル Structural analysis and characterization of lacticin Q, a novel bacteriocin belonging to a new family of unmodified bacteriocins of gram-positive bacteria.
ジャーナル Appl Environ Microbiol
Abstract Lactococcus lactis QU 5 isolated from corn produces a novel bacteriocin, termed lacticin Q. By acetone precipitation, cation-exchange chromatography, and reverse-phase high-performance liquid chromatography, lacticin Q was purified from the culture supernatant of this organism, and its molecular mass was determined to be 5,926.50 Da by mass spectrometry. Subsequent analyses of amino acid and DNA sequences revealed that lacticin Q comprised 53 amino acid residues and that its N-terminal methionine residue was formylated. In contrast to most bacteriocins produced by gram-positive bacteria, lacticin Q had no N-terminal extensions such as leader or signal sequences. It showed 66% and 48% identity to AucA, a hypothetical protein from Corynebacterium jeikeium plasmid pA501, and aureocin A53, a bacteriocin from Staphylococcus aureus A53, respectively. The characteristics of lacticin Q were determined and compared to those of nisin A. Similar to nisin A, lacticin Q exhibited antibacterial activity against various gram-positive bacteria. Lacticin Q was very stable against heat treatment and changes in pH; in particular, it was stable at alkaline pH values, while nisin A was inactivated. Moreover, lacticin Q induced ATP efflux from a Listeria sp. strain in a shorter time and at a lower concentration than nisin A, indicating that the former affected indicator cells in a different manner from that of the latter. The results described here clarified the fact that lacticin Q belongs to a new family of class II bacteriocins and that it can be employed as an alternative to or in combination with nisin A.
巻・号 73(9)
ページ 2871-7
公開日 2007-5-1
DOI 10.1128/AEM.02286-06
PII AEM.02286-06
PMID 17351096
PMC PMC1892864
MeSH Amino Acid Sequence Anti-Bacterial Agents / chemistry* Bacteriocins / genetics* Base Sequence Chromatography, High Pressure Liquid Chromatography, Ion Exchange Lactococcus lactis / genetics* Mass Spectrometry Molecular Sequence Data Nisin Sequence Analysis, DNA Sequence Homology Zea mays / microbiology*
IF 4.016
引用数 86
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM2152 JCM2504 JCM2257 JCM1465 JCM5803 JCM5804 JCM1132 JCM1095 JCM1059 JCM1134 JCM1164 JCM1157 JCM6124 JCM5885 JCM5707