RRC ID 50022
Author Kawase E, Wong MD, Ding BC, Xie T.
Title Gbb/Bmp signaling is essential for maintaining germline stem cells and for repressing bam transcription in the Drosophila testis.
Journal Development
Abstract Stem cells are responsible for replacing damaged or dying cells in various adult tissues throughout a lifetime. They possess great potential for future regenerative medicine and gene therapy. However, the mechanisms governing stem cell regulation are poorly understood. Germline stem cells (GSCs) in the Drosophila testis have been shown to reside in niches, and thus these represent an excellent system for studying relationships between niches and stem cells. Here we show that Bmp signals from somatic cells are essential for maintaining GSCs in the Drosophila testis. Somatic cyst cells and hub cells express two Bmp molecules, Gbb and Dpp. Our genetic analysis indicates that gbb functions cooperatively with dpp to maintain male GSCs, although gbb alone is essential for GSC maintenance. Furthermore, mutant clonal analysis shows that Bmp signals directly act on GSCs and control their maintenance. In GSCs defective in Bmp signaling, expression of bam is upregulated, whereas forced bam expression in GSCs causes the GSCs to be lost. This study demonstrates that Bmp signals from the somatic cells maintain GSCs, at least in part, by repressing bam expression in the Drosophila testis. dpp signaling is known to be essential for maintaining GSCs in the Drosophila ovary. This study further suggests that both Drosophila male and female GSCs use Bmp signals to maintain GSCs.
Volume 131(6)
Pages 1365-75
Published 2004-3
DOI 10.1242/dev.01025
PII dev.01025
PMID 14973292
MeSH Activin Receptors, Type II / metabolism Animals Bone Morphogenetic Proteins / metabolism* DNA-Binding Proteins / metabolism Drosophila / metabolism Drosophila Proteins / biosynthesis* Drosophila Proteins / genetics Drosophila Proteins / metabolism Male Signal Transduction / physiology Spermatogenesis / physiology* Testis / metabolism* Transcription Factors / metabolism Transcription, Genetic / physiology Transforming Growth Factor beta / metabolism*
IF 5.763
Times Cited 204
Resource
Drosophila