論文 - 詳細
| RRC ID | 50027 |
|---|---|
| 著者 | Mukai H, Takahashi M, Watanabe Y. |
| タイトル | Potential usefulness of Brevibacillus for bacterial cancer therapy: intratumoral provision of tumor necrosis factor-α and anticancer effects. |
| ジャーナル | Cancer Gene Ther |
| Abstract |
Bacterial cancer therapy, wherein bacteria are used as a gene expression system for the exogenous protein of interest in the body, has started becoming a focus area of research; therefore, studying potential bacterial species for use is extremely important. Here, we investigated the use of Brevibacillus choshinensis as an effective and safe provider of anticancer proteins in the body, using a transformant expressing murine tumor necrosis factor-α (mTNF-α). The transformant sustainably provided mTNF-α in tumors in mice for a few hours post-injection. The growth of TNF-α-sensitive tumors was inhibited even by the control transformant, which did not provide mTNF-α; intratumoral mTNF-α provision by Brevibacillus choshinensis had additive effects on tumor growth inhibition. In contrast, intratumorally injected recombinant mTNF-α did not inhibit tumor growth because of rapid elimination from the tumor. Blood biochemical and histochemical analyses showed that intravenous injection of the transformant that did not provide mTNF-α did not lead to tissue injury and dysfunction or infiltration of inflammatory cells over 1 week. Considering the findings, this approach is expected to have a high degree of usability as a delivery system for protein pharmaceuticals, especially from the viewpoints of loading capacity and cost effectiveness. |
| 巻・号 | 25(3-4) |
| ページ | 47-57 |
| 公開日 | 2018-5-1 |
| DOI | 10.1038/s41417-017-0009-7 |
| PII | 10.1038/s41417-017-0009-7 |
| PMID | 29255215 |
| MeSH | Animals Brevibacillus* / genetics Brevibacillus* / immunology Cell Line, Tumor Male Mice Mice, Inbred BALB C Mice, Nude Mice, SCID Neoplasms, Experimental* / genetics Neoplasms, Experimental* / immunology Neoplasms, Experimental* / therapy Tumor Necrosis Factor-alpha* / genetics Tumor Necrosis Factor-alpha* / immunology |
| IF | 4.534 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pBluescript SKII+mTNF alpha (RDB01478). |
| ヒト・動物細胞 | L929(RCB1422) |