論文 - 詳細
| RRC ID | 50038 |
|---|---|
| 著者 | Kanaya T, Sakakibara S, Jinnohara T, Hachisuka M, Tachibana N, Hidano S, Kobayashi T, Kimura S, Iwanaga T, Nakagawa T, Katsuno T, Kato N, Akiyama T, Sato T, Williams IR, Ohno H. |
| タイトル | Development of intestinal M cells and follicle-associated epithelium is regulated by TRAF6-mediated NF-κB signaling. |
| ジャーナル | J Exp Med |
| Abstract |
M cells are located in the follicle-associated epithelium (FAE) that covers Peyer's patches (PPs) and are responsible for the uptake of intestinal antigens. The differentiation of M cells is initiated by receptor activator of NF-κB. However, the intracellular pathways involved in M cell differentiation are still elusive. In this study, we demonstrate that the NF-κB pathway activated by RANK is essential for M cell differentiation using in vitro organoid culture. Overexpression of NF-κB transcription factors enhances the expression of M cell-associated molecules but is not sufficient to complete M cell differentiation. Furthermore, we evaluated the requirement for tumor necrosis factor receptor-associated factor 6 (TRAF6). Conditional deletion of TRAF6 in the intestinal epithelium causes a complete loss of M cells in PPs, resulting in impaired antigen uptake into PPs. In addition, the expression of FAE-associated genes is almost silenced in TRAF6-deficient mice. This study thus demonstrates the crucial role of TRAF6-mediated NF-κB signaling in the development of M cells and FAE. |
| 巻・号 | 215(2) |
| ページ | 501-519 |
| 公開日 | 2018-2-5 |
| DOI | 10.1084/jem.20160659 |
| PII | jem.20160659 |
| PMID | 29339448 |
| PMC | PMC5789402 |
| MeSH | Animals Cell Differentiation Cell Lineage Humans Intestinal Mucosa / cytology* Intestinal Mucosa / immunology* Intestinal Mucosa / metabolism Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout NF-kappa B / metabolism* Organoids / cytology Organoids / immunology Organoids / metabolism Peyer's Patches / cytology Peyer's Patches / immunology Peyer's Patches / metabolism RANK Ligand / metabolism Receptor Activator of Nuclear Factor-kappa B / metabolism Signal Transduction TNF Receptor-Associated Factor 6 / deficiency TNF Receptor-Associated Factor 6 / genetics TNF Receptor-Associated Factor 6 / metabolism* |
| IF | 10.892 |
| 引用数 | 24 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 25 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CSII-CMV-RfA-IRES2-Venus (RDB04388) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393). |