Reference - Detail
| RRC ID | 50045 |
|---|---|
| Author | Nakajima K, Tazawa I, Yaoita Y. |
| Title | Thyroid Hormone Receptor α- and β-Knockout Xenopus tropicalis Tadpoles Reveal Subtype-Specific Roles During Development. |
| Journal | Endocrinology |
| Abstract |
Thyroid hormone (TH) binds TH receptor α (TRα) and β (TRβ) to induce amphibian metamorphosis. Whereas TH signaling has been well studied, functional differences between TRα and TRβ during this process have not been characterized. To understand how each TR contributes to metamorphosis, we generated TRα- and TRβ-knockout tadpoles of Xenopus tropicalis and examined developmental abnormalities, histology of the tail and intestine, and messenger RNA expression of genes encoding extracellular matrix-degrading enzymes. In TRβ-knockout tadpoles, tail regression was delayed significantly and a healthy notochord was observed even 5 days after the initiation of tail shortening (stage 62), whereas in the tails of wild-type and TRα-knockout tadpoles, the notochord disappeared after ∼1 day. The messenger RNA expression levels of genes encoding extracellular matrix-degrading enzymes (MMP2, MMP9TH, MMP13, MMP14, and FAPα) were obviously reduced in the tail tip of TRβ-knockout tadpoles, with the shortening tail. The reduction in olfactory nerve length and head narrowing by gill absorption were also affected. Hind limb growth and intestinal shortening were not compromised in TRβ-knockout tadpoles, whereas tail regression and olfactory nerve shortening appeared to proceed normally in TRα-knockout tadpoles, except for the precocious development of hind limbs. Our results demonstrated the distinct roles of TRα and TRβ in hind limb growth and tail regression, respectively. |
| Volume | 159(2) |
| Pages | 733-743 |
| Published | 2018-2-1 |
| DOI | 10.1210/en.2017-00601 |
| PII | 4590184 |
| PMID | 29126198 |
| MeSH | Animals Gene Expression Regulation, Developmental Larva / genetics Larva / growth & development Larva / metabolism* Metalloendopeptidases / genetics Metalloendopeptidases / metabolism Tail / growth & development Tail / metabolism Thyroid Hormone Receptors alpha / genetics Thyroid Hormone Receptors alpha / metabolism* Thyroid Hormone Receptors beta / genetics Thyroid Hormone Receptors beta / metabolism* Xenopus / genetics Xenopus / growth & development* Xenopus / metabolism |
| IF | 3.934 |
| Times Cited | 19 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Clawed frogs / Newts | Xenopus tropicalis Ivory Coast |