RRC ID 50068
Author Kawai Y, Oda A, Kanai Y, Goitsuka R.
Title Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis.
Journal PLoS One
Abstract PKnox1 (also known as Prep1) belongs to the TALE family of homeodomain transcription factors that are critical for regulating growth and differentiation during embryonic and postnatal development in vertebrates. We demonstrate here that PKnox1 is required for adult spermatogenesis in a germ cell-intrinsic manner. Tamoxifen-mediated PKnox1 loss in the adult testes, as well as its germ cell-specific ablation, causes testis hypotrophy with germ cell apoptosis and, as a consequence, compromised spermatogenesis. In PKnox1-deficient testes, spermatogenesis was arrested at the c-Kit+ spermatogonia stage, with a complete loss of the meiotic spermatocytes, and was accompanied by compromised differentiation of the c-Kit+ spermatogonia. Taken together, these results indicate that PKnox1 is a critical regulator of maintenance and subsequent differentiation of the c-Kit+ stage of spermatogonia in the adult testes.
Volume 13(1)
Pages e0190702
Published 2018-1-2
DOI 10.1371/journal.pone.0190702
PII PONE-D-17-34929
PMID 29293683
PMC PMC5749842
MeSH Animals Cell Differentiation Homeodomain Proteins / physiology* Male Mice Mice, Inbred C57BL Spermatogenesis / physiology* Spermatogonia / cytology Spermatozoa / metabolism* Testis / cytology Testis / metabolism
IF 2.74
Times Cited 5
Mice RBRC01834