Reference - Detail
| RRC ID | 50108 |
|---|---|
| Author | Abe K, Takamatsu T, Sato T. |
| Title | Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis. |
| Journal | Nucleic Acids Res |
| Abstract |
A sporulation-specific gene, spsM, is disrupted by an active prophage, SPβ, in the genome of Bacillus subtilis. SPβ excision is required for two critical steps: the onset of the phage lytic cycle and the reconstitution of the spsM-coding frame during sporulation. Our in vitro study demonstrated that SprA, a serine-type integrase, catalyzed integration and excision reactions between attP of SPβ and attB within spsM, while SprB, a recombination directionality factor, was necessary only for the excision between attL and attR in the SPβ lysogenic chromosome. DNA recombination occurred at the center of the short inverted repeat motif in the unique conserved 16 bp sequence among the att sites (5΄-ACAGATAA/AGCTGTAT-3΄; slash, breakpoint; underlines, inverted repeat), where SprA produced the 3΄-overhanging AA and TT dinucleotides for rejoining the DNA ends through base-pairing. Electrophoretic mobility shift assay showed that SprB promoted synapsis of SprA subunits bound to the two target sites during excision but impaired it during integration. In vivo data demonstrated that sprB expression that lasts until the late stage of sporulation is crucial for stable expression of reconstituted spsM without reintegration of the SPβ prophage. These results present a deeper understanding of the mechanism of the prophage-mediated bacterial gene regulatory system. |
| Volume | 45(11) |
| Pages | 6669-6683 |
| Published | 2017-6-20 |
| DOI | 10.1093/nar/gkx466 |
| PII | 3848393 |
| PMID | 28535266 |
| PMC | PMC5499854 |
| MeSH | Bacillus subtilis / physiology* Bacillus subtilis / virology Bacteriophages / genetics DNA, Bacterial / genetics* Gene Expression Gene Expression Regulation, Bacterial Integrases / physiology Prophages / genetics Recombination, Genetic Repressor Proteins / physiology Spores, Bacterial / enzymology Spores, Bacterial / genetics* Spores, Bacterial / virology Virus Activation |
| IF | 11.502 |
| Times Cited | 7 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes B. subtilis | MBS550 MBS551 MBS552 |