| RRC ID |
50109
|
| Author |
Abe K, Shimizu SY, Tsuda S, Sato T.
|
| Title |
A novel non prophage(-like) gene-intervening element within gerE that is reconstituted during sporulation in Bacillus cereus ATCC10987.
|
| Journal |
Sci Rep
|
| Abstract |
Gene rearrangement is a widely-shared phenomenon in spore forming bacteria, in which prophage(-like) elements interrupting sporulation-specific genes are excised from the host genome to reconstitute the intact gene. Here, we report a novel class of gene-intervening elements, named gin, inserted in the 225 bp gerE-coding region of the B. cereus ATCC10987 genome, which generates a sporulation-specific rearrangement. gin has no phage-related genes and possesses three site-specific recombinase genes; girA, girB, and girC. We demonstrated that the gerE rearrangement occurs at the middle stage of sporulation, in which site-specific DNA recombination took place within the 9 bp consensus sequence flanking the disrupted gerE segments. Deletion analysis of gin uncovered that GirC and an additional factor, GirX, are responsible for gerE reconstitution. Involvement of GirC and GirX in DNA recombination was confirmed by an in vitro recombination assay. These results broaden the definition of the sporulation-specific gene rearrangement phenomenon: gene-intervening elements are not limited to phage DNA but may include non-viral genetic elements that carry a developmentally-regulated site-specific recombination system.
|
| Volume |
7(1)
|
| Pages |
11426
|
| Published |
2017-9-12
|
| DOI |
10.1038/s41598-017-11796-8
|
| PII |
10.1038/s41598-017-11796-8
|
| PMID |
28900282
|
| PMC |
PMC5595907
|
| MeSH |
Amino Acid Sequence
Bacillus cereus / physiology*
Bacillus cereus / virology
Bacterial Proteins / chemistry
Bacterial Proteins / genetics*
Base Sequence
Gene Order
Gene Rearrangement
Genes, Bacterial
Genetic Loci
Spores, Bacterial*
|
| IF |
3.998
|
| Times Cited |
2
|
| Resource |
| Prokaryotes B. subtilis |
MBS535
MBS536
MBS537
MBS538
MBS539
MBS540
MBS541
MBS542
MBS543
MBS544
MBS545
MBS546
MBS547
MBS548
MBS549 |
