Reference - Detail
| RRC ID | 50109 |
|---|---|
| Author | Abe K, Shimizu SY, Tsuda S, Sato T. |
| Title | A novel non prophage(-like) gene-intervening element within gerE that is reconstituted during sporulation in Bacillus cereus ATCC10987. |
| Journal | Sci Rep |
| Abstract |
Gene rearrangement is a widely-shared phenomenon in spore forming bacteria, in which prophage(-like) elements interrupting sporulation-specific genes are excised from the host genome to reconstitute the intact gene. Here, we report a novel class of gene-intervening elements, named gin, inserted in the 225 bp gerE-coding region of the B. cereus ATCC10987 genome, which generates a sporulation-specific rearrangement. gin has no phage-related genes and possesses three site-specific recombinase genes; girA, girB, and girC. We demonstrated that the gerE rearrangement occurs at the middle stage of sporulation, in which site-specific DNA recombination took place within the 9 bp consensus sequence flanking the disrupted gerE segments. Deletion analysis of gin uncovered that GirC and an additional factor, GirX, are responsible for gerE reconstitution. Involvement of GirC and GirX in DNA recombination was confirmed by an in vitro recombination assay. These results broaden the definition of the sporulation-specific gene rearrangement phenomenon: gene-intervening elements are not limited to phage DNA but may include non-viral genetic elements that carry a developmentally-regulated site-specific recombination system. |
| Volume | 7(1) |
| Pages | 11426 |
| Published | 2017-9-12 |
| DOI | 10.1038/s41598-017-11796-8 |
| PII | 10.1038/s41598-017-11796-8 |
| PMID | 28900282 |
| PMC | PMC5595907 |
| MeSH | Amino Acid Sequence Bacillus cereus / physiology* Bacillus cereus / virology Bacterial Proteins / chemistry Bacterial Proteins / genetics* Base Sequence Gene Order Gene Rearrangement Genes, Bacterial Genetic Loci Spores, Bacterial* |
| IF | 3.998 |
| Times Cited | 2 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes B. subtilis | MBS535 MBS536 MBS537 MBS538 MBS539 MBS540 MBS541 MBS542 MBS543 MBS544 MBS545 MBS546 MBS547 MBS548 MBS549 |