Reference - Detail
| RRC ID | 5017 |
|---|---|
| Author | Yamada-Onodera K, Kojima K, Takase Y, Tani Y. |
| Title | Cloning, sequence analysis, and expression in Escherichia coli of gene encoding N-Benzyl-3-pyrrolidinol dehydrogenase from Geotrichum capitatum. |
| Journal | J Biosci Bioeng |
| Abstract |
The gene encoding N-benzyl-3-pyrrolidinol dehydrogenase (DDBJ/EMBL/GenBank accession no. AB294179), a useful biocatalyst for producing (S)-N-benzyl-3-pyrrolidinol, was cloned from the genomic DNA of Geotrichum capitatum JCM 3908. The gene contained an open reading frame consisting of 1023 nucleotides corresponding to 340 amino acid residues. The subunit molecular weight was calculated to be 39,000. The predicted amino acid sequence did not have significant similarity to those of N-benzyl-3-pyrrolidinone reductases reported previously. From 30 mM N-benzyl-3-pyrrolidinone, (S)-N-benzyl-3-pyrrolidinol was obtained with a yield >99.9% and an enantiomeric excess >99.9% in 1-h and 2-h reactions without NADH addition by the resting cells of Escherichia coli HB 101 strains harboring the expression plasmids pSG-POBS and pSF-POBS that possess the glucose dehydrogenase gene and formate dehydrogenase gene as an NADH-reproducing system, respectively, besides the N-benzyl-3-pyrrolidinol dehydrogenase gene. N-Benzyl-3-pyrrolidinol dehydrogenase activity (0.56 U/mg) was observed in E. coli (pSG-POBS), which was 17-fold the specific activity observed in G. capitatum JCM 3908. |
| Volume | 104(5) |
| Pages | 379-84 |
| Published | 2007-11-1 |
| DOI | 10.1263/jbb.104.379 |
| PII | S1389-1723(07)70176-7 |
| PMID | 18086437 |
| MeSH | Alcohol Oxidoreductases / biosynthesis* Alcohol Oxidoreductases / chemistry* Alcohol Oxidoreductases / genetics Amino Acid Sequence Catalysis Cloning, Molecular Escherichia coli / genetics Fungal Proteins / biosynthesis* Fungal Proteins / chemistry* Fungal Proteins / genetics Geotrichum / enzymology* Molecular Sequence Data Plasmids / genetics Pyrroles / metabolism* Sequence Analysis, Protein |
| IF | 2.366 |
| Times Cited | 5 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM3908 |