RRC ID 50272
Author Antonaru LA, Nürnberg DJ.
Title Role of PatS and cell type on the heterocyst spacing pattern in a filamentous branching cyanobacterium.
Journal FEMS Microbiol Lett
Abstract Cell differentiation is one of the marks of multicellular organisms. Terminally specialised nitrogen-fixing cells, termed heterocysts, evolved in filamentous cyanobacteria more than 2 Gya. The development of their spacing pattern has been thoroughly investigated in model organisms such as Anabaena sp. PCC 7120. This paper focuses on the more complex, branching cyanobacterium Mastigocladus laminosus (Stigonematales). Contrary to what has been previously published, a heterocyst spacing pattern is present in M. laminosus but it varies with the age of the culture and the morphology of the cells. Heterocysts in young, narrow trichomes were more widely spaced (∼14.8 cells) than those in old, wide trichomes (∼9.4 cells). Biochemical and transgenic experiments reveal that the heterocyst spacing pattern is affected by the heterocyst inhibitor PatS. Addition of the pentapeptide RGSGR (PatS-5) to the growth medium and overexpression of patS from Anabaena sp. PCC 7120 in M. laminosus resulted in the loss of heterocyst differentiation under nitrogen deprivation. Bioinformatics investigations indicated that putative PatS sequences within cyanobacteria are highly diverse, and fall into two main clades. Both are present in most branching cyanobacteria. Despite its more complex, branching phenotype, M. laminosus appears to use a PatS-based pathway for heterocyst differentiation, a property shared by Anabaena/Nostoc.
Volume 364(15)
Published 2017-8-15
DOI 10.1093/femsle/fnx154
PII 3983256
PMID 28859320
PMC PMC5812504
MeSH Anabaena / physiology Bacterial Proteins / genetics* Bacterial Proteins / metabolism Culture Media Cyanobacteria / genetics* Cyanobacteria / metabolism* Gene Expression Regulation, Bacterial* Nitrogen / metabolism Nitrogen Fixation*
IF 1.987
Times Cited 3
Algae NIES-592 NIES-3754 NIES-3756