RRC ID 5028
Author Sekiguchi J, Nakamura T, Miyoshi-Akiyama T, Kirikae F, Kobayashi I, Augustynowicz-Kopec E, Zwolska Z, Morita K, Suetake T, Yoshida H, Kato S, Mori T, Kirikae T.
Title Development and evaluation of a line probe assay for rapid identification of pncA mutations in pyrazinamide-resistant mycobacterium tuberculosis strains.
Journal J Clin Microbiol
Abstract Resistance of Mycobacterium tuberculosis to pyrazinamide (PZA) derives mainly from mutations in the pncA gene. We developed a reverse hybridization-based line probe assay with oligonucleotide probes designed to detect mutations in pncA. The detection of PZA resistance was evaluated in 258 clinical isolates of M. tuberculosis. The sensitivity and specificity of PZA resistance obtained by this new assay were both 100%, consistent with the results of conventional PZA susceptibility testing. This assay can be used with sputa from tuberculosis patients. It appears to be reliable and widely applicable and, given its simplicity and rapid performance, will be a valuable tool for diagnostic use.
Volume 45(9)
Pages 2802-7
Published 2007-9-1
DOI 10.1128/JCM.00352-07
PII JCM.00352-07
PMID 17596354
PMC PMC2045301
MeSH Amidohydrolases / genetics* Antitubercular Agents / pharmacology Drug Resistance, Bacterial / genetics* Humans Microbial Sensitivity Tests / methods* Mutation, Missense Mycobacterium tuberculosis / genetics* Nucleic Acid Hybridization / methods* Oligonucleotide Probes / genetics Pyrazinamide / pharmacology* Sensitivity and Specificity Sputum / microbiology Tuberculosis / microbiology
IF 5.897
Times Cited 43
General Microbes JCM6379 JCM6384 JCM6364 JCM6381 JCM6383