RRC ID 50288
Author Hozumi A, Bera S, Fujiwara D, Obayashi T, Yokoyama R, Nishitani K, Aoki K.
Title Arabinogalactan Proteins Accumulate in the Cell Walls of Searching Hyphae of the Stem Parasitic Plants, Cuscuta campestris and Cuscuta japonica.
Journal Plant Cell Physiol
Abstract Stem parasitic plants (Cuscuta spp.) develop a specialized organ called a haustorium to penetrate their hosts' stem tissues. To reach the vascular tissues of the host plant, the haustorium needs to overcome the physical barrier of the cell wall, and the parasite-host interaction via the cell wall is a critical process. However, the cell wall components responsible for the establishment of parasitic connections have not yet been identified. In this study, we investigated the spatial distribution patterns of cell wall components at a parasitic interface using parasite-host complexes of Cuscuta campestris-Arabidopsis thaliana and Cuscuta japonica-Glycine max. We focused on arabinogalactan proteins (AGPs), because AGPs accumulate in the cell walls of searching hyphae of both C. campestris and C. japonica. We found more AGPs in elongated haustoria than in pre haustoria, indicating that AGP accumulation is developmentally regulated. Using in situ hybridization, we identified five genes in C. campestris that encode hyphal-expressed AGPs that belong to the fasciclin-like AGP (FLA) family, which were named CcFLA genes. Three of the five CcFLA genes were expressed in the holdfast, which develops on the Cuscuta stem epidermis at the attachment site for the host's stem epidermis. Our results suggest that AGPs are involved in hyphal elongation and adhesion to host cells, and in the adhesion between the epidermal tissues of Cuscuta and its host.
Volume 58(11)
Pages 1868-1877
Published 2017-11-1
DOI 10.1093/pcp/pcx121
PII 4092857
PMID 29016904
MeSH Arabidopsis / parasitology Cell Adhesion / physiology Cell Wall / immunology Cell Wall / metabolism Cuscuta / cytology* Cuscuta / genetics Cuscuta / metabolism Epitopes Gene Expression Regulation, Plant Host-Parasite Interactions / physiology* Mucoproteins / chemistry Mucoproteins / genetics Mucoproteins / metabolism* Plant Epidermis / cytology Plant Epidermis / metabolism Plant Proteins / chemistry Plant Proteins / genetics Plant Proteins / metabolism Plant Stems / metabolism* Soybeans / parasitology
IF 3.929
Times Cited 3
Resource
Tomato TOMJPF00001