RRC ID 5029
Author Asai Y, Makimura Y, Kawabata A, Ogawa T.
Title Soluble CD14 discriminates slight structural differences between lipid as that lead to distinct host cell activation.
Journal J. Immunol.
Abstract Soluble CD14 (sCD14) in serum is known to sensitize host cells to LPS. In the present study, the contributions of sCD14 and LPS-binding protein to a lipid A moiety from LPS preparations of periodontopathogenic Fusobacterium nucleatum sp. nucleatum were compared with that of Escherichia coli-type synthetic lipid A (compound 506). F. nucleatum lipid A was identified to be a hexa-acylated fatty acid composed of tetradecanoate (C(14)) and hexadecanoate (C(16)), similar to dodecanoate (C(12)) and C(14) in compound 506. The two lipid A specimens exhibited nearly the same reactivity in Limulus amoebocyte lysate assays, though F. nucleatum lipid A showed a weaker lethal toxicity. Both lipid A specimens showed nearly the same activities toward host cells in the absence of FBS, though compound 506 exhibited much stronger activity in the presence of FBS, sCD14, or sCD14 together with LPS-binding protein. Furthermore, native PAGE/Western immunoblot assays demonstrated that F. nucleatum lipid A had a weaker binding to sCD14 as compared with compound 506. These results suggest that sCD14 is able to discriminate the slight structural differences between these lipid As, which causes their distinct host cell activation activities.
Volume 179(11)
Pages 7674-83
Published 2007-12-1
DOI 10.4049/jimmunol.179.11.7674
PII 179/11/7674
PMID 18025213
MeSH Animals Binding Sites Carbohydrate Conformation Culture Media / chemistry Escherichia coli / chemistry Fibroblasts / chemistry Fibroblasts / drug effects Fusobacterium nucleatum / chemistry Humans Leukocytes, Mononuclear / chemistry Leukocytes, Mononuclear / drug effects Lipid A / chemistry* Lipid A / immunology Lipid A / pharmacology Lipopolysaccharide Receptors / chemistry* Lipopolysaccharide Receptors / metabolism Lipopolysaccharides / chemistry Lipopolysaccharides / pharmacology Male Mice Mice, Inbred C3H Mice, Inbred C57BL Solubility Structure-Activity Relationship
IF 4.718
Times Cited 19
General Microbes JCM8532