Reference - Detail
| RRC ID | 502 |
|---|---|
| Author | He N, Aso Y, Fujii H, Banno Y, Yamamoto K. |
| Title | In vivo and in vitro interactions of the Bombyx mori chymotrypsin inhibitor b1 with Escherichia coli. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Various chymotrypsin inhibitors occur in the hemolymph of silkworm larvae. Interaction of chymotrypsin inhibitor b1 (CI-b1) with Escherichia coli was examined from the viewpoint of action against invading bacteria. Injection of dead E. coli cells into larva reduced the CI-b1 content of the hemolymph, suggesting in vivo binding of CI-b1 to the outer membrane of the cell. Results from incubation of E. coli in cell-free hemolymph in the presence or absence of lipopolysaccharide indicated that CI-b1 is the only CI bound to E. coli and that it interacts with lipopolysaccharide. CI-b1 formed a complex with lipopolysaccharide in vitro; the value of the dissociation constant was relatively large. Inhibitory activity of CI-b1 changed insignificantly in mixture with lipopolysaccharide. CI-b1 affected the growth of E. coli but never worked lethally. CI-b1 is speculated to be a mediator that scavenges intruding bacteria rather than a direct anti-bacterial factor. This is the first report confirming that CI-b1 is a lipopolysaccharide binding protein. |
| Volume | 68(4) |
| Pages | 835-40 |
| Published | 2004-4-1 |
| DOI | 10.1271/bbb.68.835 |
| PMID | 15118311 |
| MeSH | Animals Bombyx / chemistry* Bombyx / growth & development Bombyx / metabolism Cell Division Chymotrypsin / antagonists & inhibitors* Enzyme Inhibitors / metabolism* Enzyme Inhibitors / pharmacology Escherichia coli / cytology Escherichia coli / metabolism* Hemolymph / metabolism Larva / metabolism Lipopolysaccharides / metabolism Lipopolysaccharides / pharmacology |
| IF | 1.516 |
| Times Cited | 8 |
| WOS Category | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Silkworms | p50 |