RRC ID |
5030
|
著者 |
Iwase T, Seki K, Shinji H, Mizunoe Y, Masuda S.
|
タイトル |
Development of a real-time PCR assay for the detection and identification of Staphylococcus capitis, Staphylococcus haemolyticus and Staphylococcus warneri.
|
ジャーナル |
J Med Microbiol
|
Abstract |
Staphylococcus capitis, Staphylococcus haemolyticus and Staphylococcus warneri are coagulase-negative staphylococci. Each species has different characteristics, and a difference in pathology is also seen in compromised hosts. Therefore, the development of a species-specific simple detection method for the identification of these staphylococci is important. Here, a species-specific real-time PCR assay is reported that targets the superoxide dismutase A-encoding gene of these bacteria. Primers were designed with a base that was non-complementary with regard to the other bacteria. This base was at the 3' end of the primer (3' mismatch primer) and conferred high specificity. These primers were then evaluated using real-time PCR. They reacted only with the target bacterium. In addition, stable quantitative reactions were observed when experiments were performed using genomic DNA extracted from varying numbers of staphylococci cells (10(1)-10(7) cells). These results indicate that this method is useful for the identification and quantitative analysis of S. capitis, S. haemolyticus and S. warneri.
|
巻・号 |
56(Pt 10)
|
ページ |
1346-1349
|
公開日 |
2007-10-1
|
DOI |
10.1099/jmm.0.47235-0
|
PMID |
17893172
|
MeSH |
Bacterial Proteins / genetics
Coagulase / biosynthesis
DNA Primers / genetics
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Staphylococcal Infections / microbiology
Staphylococcus / classification*
Staphylococcus / isolation & purification*
Superoxide Dismutase / genetics
|
IF |
2.156
|
引用数 |
16
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
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