RRC ID 5030
著者 Iwase T, Seki K, Shinji H, Mizunoe Y, Masuda S.
タイトル Development of a real-time PCR assay for the detection and identification of Staphylococcus capitis, Staphylococcus haemolyticus and Staphylococcus warneri.
ジャーナル J Med Microbiol
Abstract Staphylococcus capitis, Staphylococcus haemolyticus and Staphylococcus warneri are coagulase-negative staphylococci. Each species has different characteristics, and a difference in pathology is also seen in compromised hosts. Therefore, the development of a species-specific simple detection method for the identification of these staphylococci is important. Here, a species-specific real-time PCR assay is reported that targets the superoxide dismutase A-encoding gene of these bacteria. Primers were designed with a base that was non-complementary with regard to the other bacteria. This base was at the 3' end of the primer (3' mismatch primer) and conferred high specificity. These primers were then evaluated using real-time PCR. They reacted only with the target bacterium. In addition, stable quantitative reactions were observed when experiments were performed using genomic DNA extracted from varying numbers of staphylococci cells (10(1)-10(7) cells). These results indicate that this method is useful for the identification and quantitative analysis of S. capitis, S. haemolyticus and S. warneri.
巻・号 56(Pt 10)
ページ 1346-1349
公開日 2007-10-1
DOI 10.1099/jmm.0.47235-0
PMID 17893172
MeSH Bacterial Proteins / genetics Coagulase / biosynthesis DNA Primers / genetics Polymerase Chain Reaction / methods* Sensitivity and Specificity Staphylococcal Infections / microbiology Staphylococcus / classification* Staphylococcus / isolation & purification* Superoxide Dismutase / genetics
IF 2.156
引用数 16
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM2414 JCM2415 JCM2416 JCM2417 JCM2418 JCM2420 JCM2422 JCM2424 JCM2427 JCM2874 JCM7470